Anti-XIAP-associated factor 1 XAF1 Antibody Picoband®

About this product

Boster Bio Anti-XIAP-associated factor 1 XAF1 Antibody catalog # PA1218. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1218

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

XIAP associated factor-1, also known as XAF1, is a human gene. X-linked inhibitor of apoptosis (XIAP; MIM 300079) is a potent member of the IAP family. All members of this family possess baculoviral IAP (BIR) repeats, cysteine-rich domains of approximately 80 amino acids that bind and inhibit caspases. XAF1 antagonizes the anticaspase activity of XIAP and may be important in mediating apoptosis resistance in cancer cells. And alteration in XAF1 and XIAP RNA expression levels may lead to increased apoptotic resistance and proliferation due to unregulated XIAP function in cancer cells.

Synonyms

XIAP-associated factor 1; BIRC4-binding protein; XAF1; BIRC4BP, XIAPAF1

Gene Name

XAF1

Gene ID

54739

UniProt

Q6GPH4

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human XAF1.

Clonality

Polyclonal

Tissue Specificity

Widely expressed. Expression is frequently down-regulated in cancer cell lines. Isoform 5 is widely expressed. Expressed in placenta (at protein level) . .

Applications

Flow Cytometry, IF, ICC, WB

Field of Research

Apoptosis, Caspases, Cell Biology, Intracellular

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Seems to function as a negative regulator of members of the IAP (inhibitor of apoptosis protein) family. Inhibits anti- caspase activity of BIRC4. Induces cleavage and inactivation of BIRC4 independent of caspase activation. Mediates TNF-alpha- induced apoptosis and is involved in apoptosis in trophoblast cells. May inhibit BIRC4 inly by activating the mitochondrial apoptosis pathway. After translocation to mitochondra, promotes translocation of BAX to mitochondria and cytochrome c release from mitochondria. Seems to promote the redistribution of BIRC4 from the cytoplasm to the nucleus, probably independent of BIRC4 inactivation which seems to occur in the cytoplasm. The BIRC4-XAF1 complex mediates down-regulation of BIRC5/survivin; the process requires the E3 ligase activity of BIRC4. Seems to be involved in cellular sensitivity to the proapoptotic actions of TRAIL. May be a tumor suppressor by mediating apoptosis resistance of cancer cells. .

References & Citations

1. Fong, W. G.; Liston, P.; Rajcan-Separovic, E.; St. Jean, M.; Craig, C.; Korneluk, R. G. : Expression and genetic analysis of XIAP-associated factor 1 (XAF1) in cancer cell lines. Genomics 70: 113-122, 2000. 2. Liston, P.; Fong, W. G.; Kelly, N. L.; Toji, S.; Miyazaki, T.; Conte, D.; Tamai, K.; Craig, C. G.; McBurney, M. W.; Korneluk, R. G. : Identification of XAF1 as an antagonist of XIAP anti-caspase activity. Nature Cell Biol. 3: 128-133, 2001.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

34626 MW

Observed Molecular Weight

35 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

XIAP-associated factor 1

Subcellular Location

Cytoplasm. Nucleus. Mitochondrion. Found in the cytoplasm and nucleus of placental syncytiotrophoblasts. Translocates to mitochondria upon TNF-alpha treatment.

Sequence Similarities

Contains 1 TRAF-type zinc finger.

Protein Name

XIAP-associated factor 1

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.