Anti-Cytokeratin 8/KRT8 Antibody Picoband®

About this product

Boster Bio Anti-Cytokeratin 8/KRT8 Antibody catalog # PA1240. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1240

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Keratin, type II cytoskeletal 8, also known as cytokeratin-8 (CK-8) or keratin-8 (K8) is a keratin protein that is encoded in humans by the KRT8 gene. This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene.

Synonyms

Keratin, type II cytoskeletal 8; Cytokeratin-8; CK-8; Keratin-8; K8; Type-II keratin Kb8; KRT8; CYK8

Gene Name

KRT8

Gene ID

3856

UniProt

P05787

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Cytokeratin 8, different from the related rat and mouse sequences by one amino acid.

Clonality

Polyclonal

Tissue Specificity

Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity. .

Applications

Flow Cytometry, IHC, WB

Field of Research

Class I, Cytoskeleton, Cytoskeleton/ECM, Intermediate Filaments, Keratin, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. .

References & Citations

1. He, T.; Stepulak, A.; Holmstrom, T. H.; Omary, M. B.; Eriksson, J. E. : The intermediate filament protein kinase 8 is a novel cytoplasmic substrate for c-Jun N-terminal kinase. J. Biol. Chem. 277: 10767-10774, 2002. 2. Krauss, S.; Franke, W. W. : Organization and sequence of the human gene encoding cytokeratin 8. Gene 86: 241-249, 1990. 3. Yamamoto, R.; Kao, L.-C.; McKnight, C. E.; Strauss, J. F., III : Cloning and sequence of cDNA for human placental cytokeratin 8: regulation of the mRNA in trophoblastic cells by cAMP. Molec. Endocr. 4: 370-374, 1990.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

53704 MW

Observed Molecular Weight

54 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Keratin, type II cytoskeletal 8

Subcellular Location

Cytoplasm . Nucleus, nucleoplasm . Nucleus matrix .

Sequence Similarities

Belongs to the intermediate filament family.

Protein Name

Keratin, type II cytoskeletal 8

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.