Anti-TFPI2 Antibody Picoband®

About this product

Boster Bio Anti-TFPI2 Antibody catalog # PA1248. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1248

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Tissue factor pathway inhibitor 2, also known as TFPI2, is a human gene which is located at 7q22. It is an important regulator of the extrinsic pathway of blood coagulation through its ability to inhibit factor Xa and factor VIIa-tissue factor activity. After a 22-residue signal peptide, the mature TFPI2 protein contains 213 amino acids with 18 cysteines and 2 canonical N-linked glycosylation sites. The purified recombinant TFPI2 strongly inhibited the amidolytic activities of trypsin and the factor VIIa-tissue factor complex. The latter inhibition was markedly enhanced in the presence of heparin. Mouse TFPI2 mRNA is highly expressed in developing mouse placenta, as in human. And there are also high transcript levels in adult mouse liver and kidney.

Synonyms

Tissue factor pathway inhibitor 2; TFPI-2; Placental protein 5; PP5; TFPI2

Gene Name

TFPI2

Gene ID

7980

UniProt

P48307

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human TFPI2.

Clonality

Polyclonal

Tissue Specificity

Umbilical vein endothelial cells, liver, placenta, heart, pancreas, and maternal serum at advanced pregnancy.

Applications

IHC, WB

Field of Research

Cardiovascular, Cell Biology, Protease Inhibitors, Proteolysis/Ubiquitin

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

May play a role in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa. Has no effect on thrombin. .

References & Citations

1. Sprecher, C. A.; Kisiel, W.; Mathewes, S.; Foster, D. C. : Molecular cloning, expression, and partial characterization of a second human tissue-factor-pathway inhibitor. Proc. Nat. Acad. Sci. 91: 3353-3357, 1994. 2. Miyagi, Y.; Yasumitsu, H.; Mizushima, H.; Koshikawa, N.; Matsuda, Y.; Itoh, H.; Hori, T.-A.; Aoki, I.; Misugi, K.; Miyazaki, K. : Cloning of the cDNA encoding mouse PP5/TFPI-2 and mapping of the gene to chromosome 6. DNA and Cell Biology 15: 947-954, 1996.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

26934 MW

Observed Molecular Weight

51 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Tissue factor pathway inhibitor 2

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the nuclear hormone receptor family. NR1 subfamily.

Protein Name

Tissue factor pathway inhibitor 2 (TFPI-2)

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.