Anti-c-Fos Antibody Picoband®

About this product

Boster Bio Anti-c-Fos Antibody catalog # PA1318. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1318

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

The human oncogene c-fos is cellular homolog of the transforming gene of Finkel-Biskis-Jinkins (FBJ) murine osteosarcoma virus which was mapped to a single human chromosome. c-Fos is encoded by the FOS gene. FOS was the first transcription factor identified that has a critical function in regulating the development of cells destined to form and maintain the skeleton. FOS is also a major component of the activator protein-1 (AP-1) transcription factor complex, which includes members of the JUN family. c-fos is a major nuclear target for signal transduction pathways involved in the regulation of cell growth, differentiation, and transformation. Using transgenic and knockout mice, Grigoriadis et al. (1995) established a unique role for the proto-oncogene and nuclear transcription factor, Fos, in regulating the differentiation and activity of specific bone cell populations, both during normal development and in bone disease.

Synonyms

Proto-oncogene c-Fos; Cellular oncogene fos; G0/G1 switch regulatory protein 7; FOS; G0S7

Gene Name

FOS

Gene ID

2353

UniProt

P01100

Host

Rabbit

Reactivity

Rat, Mouse, Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human c-Fos, identical to the related rat and mouse sequences.

Clonality

Polyclonal

Applications

WB

Field of Research

Cancer, Cancer Susceptibility, Domain Families, Epigenetics and Nuclear Signaling, Hlh/Leucine Zipper, Oncoproteins, Oncoproteins/Suppressors, Proto-Oncogenes, Transcription, Transcription Factors

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Nuclear phosphoprotein which forms a tight but non- covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD- binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum. .

References & Citations

1. Barker, P. E.; Rabin, M.; Watson, M.; Breg, W. R.; Ruddle, F. H.; Verma, I. M. : Human c-fos oncogene mapped within chromosomal region 14q21-q31. Proc. Nat. Acad. Sci. 81: 5826-5830, 1984. 2. Saez, E.; Rutberg, S. E.; Mueller, E.; Oppenheim, H.; Smoluk, J.; Yuspa, S. H.; Spiegelman, B. M. : c-fos is required for malignant progression of skin tumors. Cell 82: 721-732, 1995. 3. Grigoriadis, A. E.; Wang, Z.-Q.; Wagner, E. F. : Fos and bone cell development: lessons from a nuclear oncogene. Trends Genet. 11: :436-441, 1995.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

40695 MW

Observed Molecular Weight

37 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Proto-oncogene c-Fos

Subcellular Location

Nucleus. Endoplasmic reticulum. Cytoplasm, cytosol. In quiescent cells, present in very small amounts in the cytosol. Following induction of cell growth, first localizes to the endoplasmic reticulum and only later to the nucleus. Localization at the endoplasmic reticulum requires dephosphorylation at Tyr-10 and Tyr-30.

Sequence Similarities

Belongs to the bZIP family. Fos subfamily.

Protein Name

Proto-oncogene c-Fos

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.