Anti-GRP94/HSP90B1 Antibody Picoband®

About this product

Boster Bio Anti-GRP94/HSP90B1 Antibody catalog # PA1340. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1340

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Heat shock protein 90kDa beta member 1  (HSP90B1), known also as  endoplasmin, GRP94, is a chaperone protein  that in humans is encoded by the  HSP90B1  gene. It is mapped to chromosome 12q23.3. This gene encodes a member of a family of adenosine triphosphate (ATP) -metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation.

Synonyms

Endoplasmin;94 kDa glucose-regulated protein; GRP-94; Heat shock protein 90 kDa beta member 1; Tumor rejection antigen 1; gp96 homolog; HSP90B1; GRP94, TRA1

Gene Name

HSP90B1

Gene ID

7184

UniProt

P14625

Host

Rabbit

Reactivity

Human, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human GRP94, identical to the related mouse and rat sequences.

Clonality

Polyclonal

Tissue Specificity

Most abundant in testis, slightly less abundant in skeletal muscle and heart, followed by prostate, thymus, ovary, small intestine, and colon. Not detected in the peripheral blood leukocytes.

Applications

IHC, IHC-F, ICC, WB

Field of Research

Cancer, Cancer Metabolism, ER Proteins, Metabolism, Metabolism Processes, Organelles, Pathways and Processes, Protein Trafficking, Response To Hypoxia, Signal Transduction, Subcellular Markers, Tags & Cell Markers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors (By similarity) . Functions in endoplasmic reticulum associated degradation (ERAD) . Has ATPase activity. .

References & Citations

1. Rebbe NF, Hickman WS, Ley TJ, Stafford DW, Hickman S (Oct 1989) . Nucleotide sequence and regulation of a human 90-kDa heat shock protein gene. J Biol Chem 264 (25) : 15006–11. 2. Chen B, Piel WH, Gui L, Bruford E, Monteiro A (Dec 2005) . The HSP90 family of genes in the human genome: insights into their divergence and evolution. Genomics 86 (6) : 627–37. 3. Entrez Gene: HSP90AB1 Heat shock protein 90kDa alpha (cytosolic), class B member 4. Chen, B., Piel, W. H., Gui, L., Bruford, E., Monteiro, A. The HSP90 family of genes in the human genome: insights into their divergence and evolution. Genomics 86: 627-637, 2005. 5. Prodromou C, Panaretou B, Chohan S, Siligardi G, O'Brien R, Ladbury JE, Roe SM, Piper PW, Pearl LH (August 2000) . The ATPase cycle of Hsp90 drives a molecular 'clamp' via transient dimerization of the N-terminal domains. EMBO J. 19 (16) : 4383–92.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

92469 MW

Observed Molecular Weight

100 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Endoplasmin

Subcellular Location

Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Sequence Similarities

Contains 1 UBA domain.

Protein Name

Endoplasmin

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.