- Catalog number
- PA1353
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Interleukin-8 (IL-8) is a chemokine produced by macrophages and other cell types such as epithelial cells. It is also synthesized by endothelial cells, which store IL-8 in their storage vesicles, the Weibel-Palade bodies. In humans, the interleukin-8 protein is encoded by the IL8 gene. Interleukin-8 (IL8) is a member of the CXC chemokine family (Hull et al., 2001) . IL-8 is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. The genes for IL8 have been co-localized on a 335-kb genomic fragment.
- Synonyms
- Interleukin-8; IL-8; C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8; Emoctakin; Granulocyte chemotactic protein 1; GCP-1; Monocyte-derived neutrophil chemotactic factor; MDNCF; Monocyte-derived neutrophil-activating peptide; MONAP; Neutrophil-activating protein 1; NAP-1; Protein 3-10C; T-cell chemotactic factor; MDNCF-a; GCP/IL-8 protein IV; IL8/NAP1 form I; Interleukin-8; (Ala-IL-8) 77; GCP/IL-8 protein II; IL-8 (1-77) ; IL8/NAP1 form II; MDNCF-b; IL-8 (5-77) ; IL-8 (6-77) ; (Ser-IL-8) 72; GCP/IL-8 protein I; IL8/NAP1 form III; Lymphocyte-derived neutrophil-activating factor; LYNAP; MDNCF-c; Neutrophil-activating factor; NAF; IL-8 (7-77) ; GCP/IL-8 protein V; IL8/NAP1 form IV; IL-8 (8-77) ; GCP/IL-8 protein VI; IL8/NAP1 form V; IL-8 (9-77) ; GCP/IL-8 protein III; IL8/NAP1 form VI; CXCL8; IL8
- Gene Name
- IL8
- Gene ID
- 3576
- UniProt
- P10145
- Host
- Rabbit
- Reactivity
- Human
- Cross Reactivity
- No cross-reactivity with other proteins
- Immunogen
- A synthetic peptide corresponding to a sequence at the C-terminus of human IL8.
- Clonality
- Polyclonal
- Tissue Specificity
- Widely expressed; lower levels in brain and lung.
- Applications
- IHC, WB
- Field of Research
- Angiogenesis, Cancer, Cardiovascular, Cytokines, Immunology, Innate Immunity, Interleukins, Invasion/Microenvironment
- Purification
- Immunogen affinity purified.
- Concentration
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Function
- IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8 (6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8 (5-77) has increased activity on neutrophil activation and IL-8 (7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8 (1-77), respectively. .
- References & Citations
- 1. Wolff B, Burns AR, Middleton J, Rot A (November 1998) . Endothelial cell memory of inflammatory stimulation: human venular endothelial cells store interleukin 8 in Weibel-Palade bodies. 2. Utgaard JO, Jahnsen FL, Bakka A, Brandtzaeg P, Haraldsen G (November 1998) . Rapid secretion of prestored interleukin 8 from Weibel-Palade bodies of microvascular endothelial cells. 3. Modi WS, Dean M, Seuanez HN, Mukaida N, Matsushima K, O'Brien SJ (January 1990) . Monocyte-derived neutrophil chemotactic factor (MDNCF/IL-8) resides in a gene cluster along with several other members of the platelet factor 4 gene superfamily. 4. Entrez Gene: IL8 interleukin 8. 5. Köhidai L, Csaba G (July 1998) . Chemotaxis and chemotactic selection induced with cytokines (IL-8, RANTES and TNF-alpha) in the unicellular Tetrahymena pyriformis. 6. O'Donovan, N., Galvin, M., Morgan, J. G. Physical mapping of the CXC chemokine locus on human chromosome 4. Cytogenet.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 11098 MW
- Observed Molecular Weight
- 60 kDa
- Specificity
- No cross reactivity with other proteins.
- Gene Name Synonym
- Interleukin-8
- Subcellular Location
- Secreted.
- Sequence Similarities
- Belongs to the intercrine alpha (chemokine CxC) family.
- Protein Name
- Interleukin-8 (IL-8)
- Isotype
- Rabbit IgG
- Contents
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
