Anti-HSD11B1 Antibody Picoband®

About this product

Boster Bio Anti-HSD11B1 Antibody catalog # PA1372-1. Tested in IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1372-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

11beta-hydroxysteroid dehydrogenase type 1 is an NADPH-dependent enzyme highly expressed in key metabolic tissues including liver, adipose tissue, and the central nervous system. In these tissues, HSD11B1 reduces cortisone to the active hormone cortisol that activates glucocorticoid receptors. It is inhibited by carbenoxolone, a drug typically used in the treatment of peptic ulcers. Tannin et al. (1991) localized the HSD11B1 gene to chromosome 1. The localization was confirmed by isolating the gene from a chromosome 1-specific library using the cDNA as a probe. Schutte et al. (2000) mapped the HSD11B1 gene to 1q32-q41.

Synonyms

Corticosteroid 11-beta-dehydrogenase isozyme 1;1.1.1.146;11-beta-hydroxysteroid dehydrogenase 1;11-DH;11-beta-HSD1; Short chain dehydrogenase/reductase family 26C member 1; HSD11B1; HSD11, HSD11L, SDR26C1

Gene Name

HSD11B1

Gene ID

3290

UniProt

P28845

Host

Rabbit

Reactivity

Human, Monkey, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human HSD11B1, different from the related mouse sequence by two amino acids, and from the related rat sequence by four amino acids.

Clonality

Polyclonal

Tissue Specificity

Widely expressed. Highest expression in liver.

Applications

IHC, WB

Field of Research

Endocrine Metabolism, Hormone Biosynthesis, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism of Lipids and Lipoproteins, Mitochondrial, Mitochondrial Markers, Mitochondrial Metabolism, Pathways and Processes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one ion, from 7- ketocholesterol to 7-beta-hydroxycholesterol (By similarity) . .

References & Citations

1. Entrez Gene: HSD11B1 hydroxysteroid (11-beta) dehydrogenase 1.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

32401 MW

Observed Molecular Weight

36 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Corticosteroid 11-beta-dehydrogenase isozyme 1

Subcellular Location

Endoplasmic reticulum membrane ; Single-pass type II membrane protein .

Sequence Similarities

Belongs to the connexin family. Beta-type (group I) subfamily.

Protein Name

Corticosteroid 11-beta-dehydrogenase isozyme 1

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.