Anti-Fractalkine CX3CL1 Antibody Picoband®

About this product

Boster Bio Anti-Fractalkine CX3CL1 Antibody catalog # PA1401. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1401

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Chemokine (C-X3-C motif) ligand 1, also known as CX3CL1, is a large cytokine protein. It is said to be the only known member of the CX3C chemokine family. It is also commonly known under the names fractalkine (in humans) and neurotactin (in mice) . Its gene is mapped to the long arm of chromosome 8. Being the large protein, it has got 373 amino acids. Imai et al. (1997) identified a 7-transmembrane high-affinity receptor (CX3CR1; 601470) for fractalkine and showed that it mediates both the adhesive and migratory functions of fractalkine.

Synonyms

Fractalkine; C-X3-C motif chemokine 1; CX3C membrane-anchored chemokine; Neurotactin; Small-inducible cytokine D1; Processed fractalkine; Cx3cl1; Cx3c, Fkn, Scyd1

Gene Name

CX3CL1

Gene ID

20312

UniProt

O35188

Host

Rabbit

Reactivity

Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of mouse CX3CL1, identical to the related rat sequence.

Clonality

Polyclonal

Tissue Specificity

Highest levels in brain. Lower levels in kidney, heart and lung. Also found in skeletal muscle and testis.

Applications

IHC, WB

Field of Research

Atherosclerosis, Cardiovascular, Chemokines, Immunology, Innate Immunity, Macrophage/Inflammation, Vascular Inflammation

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium (By similarity) . Binds to CX3CR1. .

References & Citations

1. Pan et al., Lloyd C, Zhou H, Dolich S, Deeds J, Gonzalo JA, Vath J, Gosselin M et al. (1997) . Neurotactin, a membrane-anchored chemokine upregulated in brain inflammation. Nature 387 (6633) : 611–617. doi:10.1038/42491. PMID 9177350. 2. Bazan et al., Bacon KB, Hardiman G, Wang W, Soo K, Rossi D, Greaves DR, Zlotnik A et al. (1997) . A new class of membrane-bound chemokine with a CX3C motif. Nature 385 (6617) : 640–644. doi:10.1038/385640a0. PMID 9024663. 3. Hannan, N. J., Jones, R. L., Critchley, H. O. D., Kovacs, G. J., Rogers, P. A. W., Affandi, B., Salamonsen, L. A.Coexpression of fractalkine and its receptor in normal human endometrium and in endometrium from users of progestin-only contraception supports a role of fractalkine in leukocyte recruitment and endometrial remodeling.J. Clin. Endocr. Metab.89: 6119-6129, 2004.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

42099 MW

Observed Molecular Weight

90-100 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Fractalkine

Subcellular Location

Cell membrane; Single-pass type I membrane protein.

Protein Name

Fractalkine

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.