Anti-Progesterone Receptor/PGR Antibody Picoband®

About this product

Boster Bio Anti-Progesterone Receptor/PGR Antibody catalog # PA1413. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1413

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

The progesterone receptor (PR) is an intracellular steroid receptor that specially binds progesterone in humans. PR has been a member of the steroid receptor superfamily. It is encoded by a single PGR gene residing on chromosome 11q22. The PGR gene uses separate promoters and translational start sites to produce 2 isoforms, PRA and PRB, which are identical except for an additional 165 amino acids present only in the N terminus of PRB. It can be observed in human breast tissues. The proteins function as dimeric molecules in nuclei to regulate the transcription of target genes in a ligand-responsive manner.

Synonyms

Progesterone receptor; PR; Nuclear receptor subfamily 3 group C member 3; PGR; NR3C3

Gene Name

PGR

Gene ID

5241

UniProt

P06401

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Progesterone Receptor, identical to the related rat and mouse sesquences.

Clonality

Polyclonal

Tissue Specificity

Highly expressed in placenta, lung, skeletal muscle and brain. Weakly expressed in brain, kidney and liver. In placenta, expressed on the apical plasma membrane of endothelium. In lung, expressed in alveolar epithelium. Up-regulated in colorectal tumors and expressed in 25% of early oral squamous cell carcinomas. .

Applications

IHC, WB

Field of Research

Cancer, Domain Families, Endocrine System, Epigenetics and Nuclear Signaling, Neuroscience, Nuclear Hormone Receptors, Nuclear Receptors, Nuclear Signaling Pathways, Signal Transduction, Signaling Pathway, Transcription, Tumor Biomarkers, Zinc Finger

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.

References & Citations

1. Misrahi M, Atger M, d'Auriol L, Loosfelt H, Meriel C, Fridlansky F, Guiochon-Mantel A, Galibert F, Milgrom E (March 1987) . Complete amino acid sequence of the human progesterone receptor deduced from cloned cDNA. Biochem. Biophys. Res. Commun. 143 (2) : 740–8. 2. Law ML, Kao FT, Wei Q, Hartz JA, Greene GL, Zarucki-Schulz T, Conneely OM, Jones C, Puck TT, O'Malley BW (May 1987) . The progesterone receptor gene maps to human chromosome band 11q13, the site of the mammary oncogene int-2. Proc. Natl. Acad. Sci. U.S.A. 84 (9) : 2877–81. doi:10.1073/pnas.84.9.2877. PMC 304763. PMID 3472240. 3. Nishihara T, Kitaura M, Imagawa M, Nishikawa J (1995) . Vitamin D receptor contains multiple dimerization interfaces that are functionally different. Nucleic Acids Res. 23 (4) : 606–611. 4. Schmitt J, De Vos P, Verhoeven G, Stunnenberg HG (1994) . Human androgen receptor expressed in HeLa cells activates transcription in vitro. Nucleic Acids Res. 22 (7) : 1161–1166.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

98981 MW

Observed Molecular Weight

36 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Progesterone receptor

Subcellular Location

Nucleus. Cytoplasm. Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G (1) and G (2) /M phases.

Sequence Similarities

Belongs to the nuclear hormone receptor family. NR3 subfamily.

Protein Name

Progesterone receptor (PR)

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.