Anti-Galectin 1/LGALS1 Antibody Picoband®

About this product

Boster Bio Anti-Galectin 1/LGALS1 Antibody catalog # PA1422. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1422

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Galectin-1 is a protein that in humans is encoded by the LGALS1 gene. Galectins belong to a family of related beta-galactoside-binding lectins, also referred to as S-type or S-Lac lectins. Its gene is mapped to 22q12-q13. Galectin-1 was expressed in a subset of slowly dividing subventricular zone astrocytes, which included the neural stem cells. It has been considered as an autocrine negative growth factor that regulates cell proliferation.

Synonyms

Galectin-1; Gal-1;14 kDa laminin-binding protein; HLBP14;14 kDa lectin; Beta-galactoside-binding lectin L-14-I; Galaptin; HBL; HPL; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; Putative MAPK-activating protein PM12; S-Lac lectin 1; LGALS1

Gene Name

LGALS1

Gene ID

3956

UniProt

P09382

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Galectin 1, different from the related rat and mouse sequences by one amino acid.

Clonality

Polyclonal

Tissue Specificity

Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level) . Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells. .

Applications

Flow Cytometry, IF, IHC, IHC-F, ICC, WB

Field of Research

Cell Biology

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis. .

References & Citations

1. Gitt MA, Barondes SH (February 1991) . Genomic sequence and organization of two members of a human lectin gene family. Biochemistry 30 (1) : 82–9. 2. Gauthier L, Rossi B, Roux F, Termine E, Schiff C (October 2002) . Galectin-1 is a stromal cell ligand of the pre-B cell receptor (BCR) implicated in synapse formation between pre-B and stromal cells and in pre-BCR triggering. Proc Natl Acad Sci U S A 99 (20) : 13014–9. 3. Mehrabian, M., Gitt, M. A., Sparkes, R. S., Leffler, H., Barondes, S. H., Lusis, A. J.Two members of the S-lac lectin gene family, LGALS1 and LGALS2, reside in close proximity on human chromosome 22q12-q13.Genomics15: 418-420, 1993.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

14716 MW

Observed Molecular Weight

15 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Galectin-1

Subcellular Location

Secreted, extracellular space, extracellular matrix .

Sequence Similarities

Contains 1 galectin domain.

Protein Name

Galectin-1 (Gal-1)

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.01mg NaN3.