Anti-Aquaporin 3/AQP3 Antibody Picoband®

About this product

Boster Bio Anti-Aquaporin 3/AQP3 Antibody catalog # PA1488. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1488

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene encodes the water channel protein aquaporin 3. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein, also known as aquaporin 0. Aquaporin 3 is localized at the basal lateral membranes of collecting duct cells in the kidney. In addition to its water channel function, aquaporin 3 has been found to facilitate the transport of nonionic small solutes such as urea and glycerol, but to a smaller degree. It has been suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.

Synonyms

Aquaporin-3; AQP-3; Aquaglyceroporin-3; AQP3

Gene Name

AQP3

Gene ID

360

UniProt

Q92482

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 3, different from the related rat and mouse sequences by one amino acid.

Clonality

Polyclonal

Tissue Specificity

Widely expressed in epithelial cells of kidney (collecting ducts) and airways, in keratinocytes, immature dendritic cells and erythrocytes. Isoform 2 is not detectable in erythrocytes at the protein level.

Applications

Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Cancer, Channels, Metabolism, Plasma Membrane, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Water channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the ion of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism (By similarity) . .

References & Citations

1. Dibas AI, Mia AJ, Yorio T (1998) . Aquaporins (water channels) : role in vasopressin-activated water transport. Proc. Soc. Exp. Biol. Med. 219 (3) : 183–99. 2. Roudier N, Ripoche P, Gane P, Le Pennec PY, Daniels G, Cartron JP, Bailly P (2002) . AQP3 deficiency in humans and the molecular basis of a novel blood group system, GIL. J. Biol. Chem. 277 (48) : 45854–9. 3. Sasaki S, Ishibashi K, Marumo F (1998) . Aquaporin-2 and -3: representatives of two subgroups of the aquaporin family colocalized in the kidney collecting duct. Annu. Rev. Physiol. 60: 199–220.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

31544 MW

Observed Molecular Weight

32-36 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Aquaporin-3

Subcellular Location

Basolateral cell membrane; Multi-pass membrane protein. In collecting ducts of kidney.

Sequence Similarities

Belongs to the MIP/aquaporin (TC 1.A.8) family.

Protein Name

Aquaporin-3

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.