Anti-Caspase 4/CASP4 Antibody Picoband®

About this product

Boster Bio Anti-Caspase 4/CASP4 Antibody catalog # PA1490. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1490

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Caspase 4 is an enzyme that proteolytically cleaves other proteins at an aspartic acid residue, and belongs to a family of cysteine proteases called caspases. The Caspase 4 gene is mapped to a P1 clone containing the ICE gene, which is located at chromosome 11q22.2-q22.3. It contains 8 coding exons.The function of caspase 4 is not fully known, but it is believed to be an inflammatory caspase, along with caspase 1, caspase 5 (and the murine homolog caspase 11), with a role in the immune system.

Synonyms

Caspase-4; CASP-4;3.4.22.57; ICE (rel) -II; Protease ICH-2; Protease TX; Caspase-4 subunit 1; Caspase-4 subunit 2; CASP4; ICH2

Gene Name

CASP4

Gene ID

837

UniProt

P49662

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Caspase 4.

Clonality

Polyclonal

Tissue Specificity

Widely expressed, with highest levels in spleen and lung. Moderate expression in heart and liver, low expression in skeletal muscle, kidney and testis. Not found in the brain.

Applications

IHC, WB

Field of Research

Apoptosis, Apoptotic Markers, Cancer, Caspases, Cell Biology, Cell Death, Intracellular, Invasion/Microenvironment, Metabolism, Metabolism Processes, Pathways and Processes

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Involved in the activation cascade of caspases responsible for apoptosis execution. Involved in ER-stress induced apoptosis. Cleaves caspase-1. .

References & Citations

1. Kajiwara, Y., Akram, A., Katsel, P., Haroutunian, V., Schmeidler, J., Beecham, G., Haines, J. L., Pericak-Vance, M. A., Buxbaum, J. D.FE65 binds teashirt, inhibiting expression of primate-specific caspase-4. PLoS One 4:e5071, 2009. 2. Kamens, J., Paskind, M., Hugunin, M., Talanian, R. V., Allen, H., Banach, D., Bump, N., Hackett, M., Johnston, C. G., Li, P., Mankovich, J. A., Terranova, M., Ghayur, T.Identification and characterization of ICH-2, a novel member of the interleukin-1-beta-converting enzyme family of cysteine proteases.J. Biol. Chem. 270: 15250-15256, 1995.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

43262 MW

Observed Molecular Weight

20 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Caspase-4

Subcellular Location

Endoplasmic reticulum membrane. Mitochondrion.

Sequence Similarities

Belongs to the peptidase C14A family.

Protein Name

Caspase-4

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.