Anti-CaMKK/CAMKK1 Antibody Picoband®

About this product

Boster Bio Anti-CaMKK/CAMKK1 Antibody catalog # PA1520. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1520

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

CAMKK1, Calcium/calmodulin-dependent protein kinase kinase 1 is an enzyme that in humans is encoded by the CAMKK1 gene. The CAMKK1 gene is mapped to chromosome 17. The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca (2+) /calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade. Three transcript variants encoding two distinct isoforms have been identified for this gene.Camkk1binds calmodulin and activated Camk4 with a 6-fold increase in total activity and a 100-fold increase in Camk4 Ca (2+) -independent activity. Camkk1 catalyzed a 10-fold increase in the total activity of Camk1 and had no effect on Camk2. Cotransfection of COS-7 cells with Camkk1 and Camk4 resulted in a 14-fold increase in CRE-binding protein-dependent transcription compared with Camk4 alone, suggesting that Camkk1 enhances Camk4-mediated transcriptional regulation.

Synonyms

Calcium/calmodulin-dependent protein kinase kinase 1; CaM-KK 1; CaM-kinase kinase 1; CaMKK 1;2.7.11.17; CaM-kinase IV kinase; Calcium/calmodulin-dependent protein kinase kinase alpha; CaM-KK alpha; CaM-kinase kinase alpha; CaMKK alpha; Camkk1; Camkk

Gene Name

CAMKK1

Gene ID

55984

UniProt

Q8VBY2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of mouse CaMKK, different from the related human sequence by three amino acids, and from the related rat sequence by two amino acids.

Clonality

Polyclonal

Tissue Specificity

Widely expressed. Differentially expressed in various brain regions. .

Applications

IHC, WB

Field of Research

Calcium Signaling, Calmodulin Pathway, Neuroscience, Neurotransmission, Signal Transduction, Signaling Pathway

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Calcium/calmodulin-dependent protein kinase that belongs to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Phosphorylates CAMK1, CAMK1D, CAMK1G and CAMK4. Involved in regulating cell apoptosis. Promotes cell survival by phosphorylating AKT1/PKB that inhibits pro-apoptotic BAD/Bcl2-antagonist of cell death. .

References & Citations

1. Blaeser, F., Sanders, M. J., Truong, N., Ko, S., Wu, L. J., Wozniak, D. F., Fanselow, M. S., Zhuo, M., Chatila, T. A.Long-term memory deficits in pavlovian fear conditioning in Ca (2+) /calmodulin kinase kinase alpha-deficient mice.Molec. Cell. Biol. 26: 9105-9115, 2006. 2. Tokumitsu, H., Enslen, H., Soderling, T. R.Characterization of a Ca (2+) /calmodulin-dependent protein kinase cascade: molecular cloning and expression of calcium/calmodulin-dependent protein kinase kinase.J. Biol. Chem. 270: 19320-19324, 1995.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

55838 MW

Observed Molecular Weight

56 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Calcium/calmodulin-dependent protein kinase kinase 1

Subcellular Location

Cytoplasm . Nucleus .

Sequence Similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Protein Name

Calcium/calmodulin-dependent protein kinase kinase 1 (CaM-KK 1/CaM-kinase kinase 1/CaMKK 1)

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.