Anti-BCAT1 Antibody Picoband®

About this product

Boster Bio Anti-BCAT1 Antibody catalog # PA1538. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1538

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

BCAT1, Branched-chain Aminotransferase1, is also know as BCT1. The BCAT1 gene is highly expressed early in embryogenesis, and during organogenesis its expression is localized to the neural tube, the somites, and the mesonephric tubules. The gene is also expressed in several MYC-based tumors. The BCAT1 gene is mapped to chromosome 12. Lack of the enzyme BCT can cause auxotroph, a kind of auxotrophic mutant in Chinese-hamster ovary cells that lacks the ability to grow if alpha-ketoisovaleric acid, alpha-ketoisocaproic acid, and alpha-keto-beta-methylvaleric acid are substituted for valine, leucine, and isoleucine in the culture medium.

Synonyms

Branched-chain-amino-acid aminotransferase, cytosolic; BCAT (c) ;2.6.1.42; Protein ECA39; BCAT1; BCT1, ECA39

Gene Name

BCAT1

Gene ID

586

UniProt

P54687

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human BCAT1, different from the related mouse sequence by one amino acid, rat sequence by three amino acids.

Clonality

Polyclonal

Tissue Specificity

During embryogenesis, expressed in the brain and kidney. Overexpressed in MYC-induced tumors such as Burkitt's lymphoma.

Applications

Flow Cytometry, IHC, WB

Field of Research

Amino Acid Metabolism, Amino Acids, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine.

References & Citations

1. Naylor, S. L., Shows, T. B.Branched-chain aminotransferase genes (BCT-1 and BCT-2) assigned to human chromosomes 12 and 19 using alpha-keto acid selection media. (Abstract) Cytogenet. Cell Genet. 25: 191-192, 1979. 2. Jones, C., Moore, E. E.Isolation of mutants lacking branched-chain amino acid transaminase.Somat. Cell Genet. 2: 235-243, 1976.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

43 kDa

Observed Molecular Weight

43 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Branched-chain-amino-acid aminotransferase, cytosolic

Subcellular Location

Cytoplasm.

Sequence Similarities

Belongs to the bZIP family.

Protein Name

Branched-chain-amino-acid aminotransferase, cytosolic (BCAT (c) )

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.