Anti-c-Myc Antibody Picoband®

About this product

Boster Bio Anti-c-Myc Antibody catalog # PA1555. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1555

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

C-Myc is an oncogene that functions both in the stimulation of cell proliferation and in apoptosis. c-Myc elicits its oncogenic activity by causing immortalization, and to a lesser extent the transformation of cells, in addition to several other mechanisms. The c-MYC proto-oncogene encodes a transcription factor that is critical for cell growth and proliferation. It is one of the genes frequently altered in cancer cells in which it exhibits constitutive activity. Downregulation of c-Myc is critical for 2-Methoxyestradiol (2ME2) -induced oxidative stress and apoptosis in AML cells. And its up-regulation is important for promoting lymphocyte cell division, and demonstrating that GFP-c-Myc expression is a marker of proliferating lymphocytes in vivo.

Synonyms

Myc proto-oncogene protein; Class E basic helix-loop-helix protein 39; bHLHe39; Proto-oncogene c-Myc; Transcription factor p64; MYC; BHLHE39

Gene Name

MYC

Gene ID

4609

UniProt

P01106

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human c-Myc, different from the related mouse and rat sequences by one amino acid.

Clonality

Polyclonal

Applications

WB

Field of Research

Cancer, Cell Cycle, Cell Differentiation, Domain Families, Epigenetics and Nuclear Signaling, Hlh/Leucine Zipper, Nuclear, Oncoproteins, Oncoproteins/Suppressors, Signaling Pathways, Stem Cells, TGF Beta, Transcription, Transcription Factors, Tumor Biomarkers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'- CAC[GA]TG-3'. Activates the transcription of growth-related genes.

References & Citations

1. Liao D, Thakur A, Wu J, Biliran H, Sarkar FH. Perspectives on c-Myc, Cyclin D1, and Their Interaction in Cancer Formation, Progression, and Response to Chemotherapy.Crit Rev Oncog. 2007;13 (2) :93-158. 2. Britton S, Salles B, Calsou P. c-MYC protein is degraded in response to UV irradiation.Cell Cycle. 2007 Oct 2;7 (1) 3. Chow JM, Liu CR, Lin CP, Lee CN, Cheng YC, Lin S, Liu HE. Downregulation of c-Myc determines sensitivity to 2-methoxyestradiol-induced apoptosis in human acute myeloid leukemia.Exp Hematol. 2008 Feb;36 (2) :140-8. 4. Huang CY, Bredemeyer AL, Walker LM, Bassing CH, Sleckman BP. Dynamic regulation of c-Myc proto-oncogene expression during lymphocyte development revealed by a GFP-c-Myc knock-in mouse.Eur J Immunol. 2008 Jan 14.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

48804 MW

Observed Molecular Weight

49 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Myc proto-oncogene protein

Subcellular Location

Nucleus, nucleoplasm . Nucleus, nucleolus .

Sequence Similarities

Contains 1 bHLH (basic helix-loop-helix) domain.

Protein Name

Myc proto-oncogene protein

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.