Anti-Histone deacetylase 3 HDAC3 Antibody Picoband®

About this product

Boster Bio Anti-Histone deacetylase 3 HDAC3 Antibody catalog # PA1600-1. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1600-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

HDAC3 (HISTONE DEACETYLASE 3) is a member of the histone deacetylase/acuc/apha family of proteins that is an enzyme that in humans is encoded by the HDAC3 gene. The HDAC3 gene is mapped to 5q31.3. HDAC3 has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. The protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. And this gene is regarded as a potential tumor suppressor gene. HDAC3 has an open reading frame of 428 amino acids and shares 53% amino acid identity with HDAC1 and 52% with HDAC2. The catalytic domain of HDAC4 interacts with HDAC3 via the transcriptional corepressor NCOR2. All experimental conditions leading to the suppression of HDAC4 binding to NCOR2 and to HDAC3 resulted in loss of enzymatic activity associated with HDAC4. HDAC3 recruitment to the genome displays a circadian rhythm in mouse liver.

Synonyms

Histone deacetylase 3; HD3;3.5.1.98; RPD3-2; SMAP45; HDAC3

Gene Name

HDAC3

Gene ID

8841

UniProt

O15379

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human HDAC3, identical to the related rat and mouse sequences.

Clonality

Polyclonal

Tissue Specificity

Widely expressed.

Applications

Flow Cytometry, IF, IHC, IHC-F, ICC, WB

Field of Research

Lipid and Lipoprotein Metabolism, Lipid Metabolism, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism of Lipids and Lipoproteins, Oncoproteins/Suppressors, Pathways and Processes, Signaling Pathways, Stem Cells, Tumor Suppressors

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Participates in the BCL6 transcriptional repressor activity by deacetylating the H3 'Lys- 27' (H3K27) on enhancer elements, antagonizing EP300 acetyltransferase activity and repressing proximal gene expression. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation (PubMed:21444723, PubMed:23911289) . Contributes, together with XBP1 isoform 1, to the activation of NFE2L2-mediated HMOX1 transcription factor gene expression in a PI (3) K/mTORC2/Akt-dependent signaling pathway leading to endothelial cell (EC) survival under disturbed flow/oxidative stress (PubMed:25190803) . .

References & Citations

1. Alenghat, T., Meyers, K., Mullican, S. E., Leitner, K., Adeniji-Adele, A., Avila, J., Bucan, M., Ahima, R. S., Kaestner, K. H., Lazar, M. A. Nuclear receptor corepressor and histone deacetylase 3 govern circadian metabolic physiology. Nature 456: 997-1000, 2008. 2. Chen, L., Fischle, W., Verdin, E., Greene, W. C. Duration of nuclear NF-kappa-B action regulated by reversible acetylation. Science 293: 1653-1657, 2001. 3. Dangond F, Hafler DA, Tong JK, Randall J, Kojima R, Utku N, Gullans SR (Mar 1998) . Differential display cloning of a novel human histone deacetylase (HDAC3) cDNA from PHA-activated immune cells. Biochem Biophys Res Commun 242 (3) : 648–52. doi:10.1006/bbrc.1997.8033.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

48848 MW

Observed Molecular Weight

45 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Histone deacetylase 3

Subcellular Location

Nucleus . Cytoplasm . Cytoplasm, cytosol . Colocalizes with XBP1 and AKT1 in the cytoplasm (PubMed:25190803) . Predominantly expressed in the nucleus in the presence of CCAR2. .

Sequence Similarities

Belongs to the histone deacetylase family. HD type 1 subfamily.

Protein Name

Histone deacetylase 3 (HD3)

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.