Anti-Hsp105/HSPH1 Antibody Picoband®

About this product

Boster Bio Anti-Hsp105/HSPH1 Antibody catalog # PA1608. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1608

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

HSP105 (HEAT-SHOCK 105/110-KD PROTEIN 1), also called HSPH1 or HSP110, is a protein that in humans is encoded by the HSPH1 gene. Immunohistochemical analysis localizes HSP105 mainly in the cytoplasm. Database analysis indicates that both HSP105 isoforms are highly conserved during evolution. By analysis of radiation hybrids and human/rodent hybrid cell lines, the HSPH1 gene is mapped to chromosome 13. Both HSP105-alpha and HSP105-beta are upregulated in HeLa cells exposed to heat shock. HSP105-alpha, but not HSP105-beta, is also upregulate in response to other cell stresses. Following heat shock, HSP105 relocalizes from a cytoplasmic to perinuclear position. Besides, HSP110 may thus constitute a major determinant for both prognosis and treatment response in colorectal cancer.

Synonyms

Heat shock protein 105 kDa; Antigen NY-CO-25; Heat shock 110 kDa protein; HSPH1; HSP105, HSP110, KIAA0201

Gene Name

HSPH1

Gene ID

10808

UniProt

Q92598

Host

Rabbit

Reactivity

Rat, Mouse, Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Hsp105, different from the related mouse sequence by one amino acid, and different from the related rat sequence by two amino acids.

Clonality

Polyclonal

Tissue Specificity

Highly expressed in testis. Present at lower levels in most brain regions, except cerebellum. Overexpressed in cancer cells. .

Applications

Flow Cytometry, IF, IHC, IHC-F, ICC, WB

Field of Research

Chaperones, Heat Shock Proteins, Protein Trafficking, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Prevents the aggregation of denatured proteins in cells under severe stress, on which the ATP levels decrease markedly. Inhibits HSPA8/HSC70 ATPase and chaperone activities (By similarity) . .

References & Citations

1. Ishihara, K., Yasuda, K., Hatayama, T. Molecular cloning, expression and localization of human 105 kDa heat shock protein, hsp105. Biochim. Biophys. Acta 1444: 138-142, 1999. 2. Dorard, C., de Thonel, A., Collura, A., Marisa, L., Svrcek, M., Lagrange, A., Jego, G., Wanherdrick, K., Joly, A. L., Buhard, O., Gobbo, J., Penard-Lacronique, V., and 16 others. Expression of a mutant HSP110 sensitizes colorectal cancer cells to chemotherapy and improves disease prognosis. Nature Med. 17: 1283-1289, 2011.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

96865 MW

Observed Molecular Weight

60 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Heat shock protein 105 kDa

Subcellular Location

Cytoplasm .

Sequence Similarities

Belongs to the heat shock protein 70 family.

Protein Name

Heat shock protein 105 kDa

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.