Anti-Integrin beta 3/ITGB3 Antibody Picoband®

About this product

Boster Bio Anti-Integrin beta 3/ITGB3 Antibody catalog # PA1627. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1627

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

ITGB3 (INTEGRIN, BETA-3), also called GP3A, GPIIIa, CD61, is a protein that in humans is encoded by the ITGB3 gene. GP3A is a cluster of differentiation found on thrombocytes. The ITGB3 complex belongs to the integrin class of cell adhesion molecule receptors that share a common heterodimeric structure with alpha and beta subunits. The GP3A gene is mapped to 17q21.32. And the GP3A gene has 14 exons. The 3-prime exon is larger than 1,700 nucleotides and contains the 3-prime untranslated region. The ITGB3 complex mediates platelet aggregation by acting as a receptor for fibrinogen. Although the ITGB3 is expressed on the cell surface at normal levels and is capable of function following extracellular stimulation, it could not be activated via the inside-out signaling pathways.

Synonyms

Integrin beta-3; Platelet membrane glycoprotein IIIa; GPIIIa; CD61; ITGB3; GP3A

Gene Name

ITGB3

Gene ID

3690

UniProt

P05106

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Integrin beta 3, identical to the related rat and mouse sequences.

Clonality

Polyclonal

Tissue Specificity

Isoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis.

Applications

IHC, WB

Field of Research

Cell Adhesion, Cell Cycle, Chromosome Structure, Co-Activators/Co-Repressors, Cytoskeleton/ECM, Epigenetics and Nuclear Signaling, Integrins, NFKB Pathway, Nuclear Hormone Receptors, Nuclear Receptors, Nuclear Signaling Pathways, Signal Transduction, Signaling Pathway

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Integrin alpha-V/beta-3 (ITGAV:ITGB3) is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 (ITGA2B:ITGB3) is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha- IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha- IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. Fibrinogen binding enhances SELP expression in activated platelets (By similarity) . In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. .

References & Citations

1. Andre, P., Srinivasa Prasad, K. S., Denis, C. V., He, M., Papalia, J. M., Hynes, R. O., Phillips, D. R., Wagner, D. D. CD40L stabilizes arterial thrombi by a beta (3) integrin-dependent mechanism. Nature Med. 8: 247-252, 2002. 2. Anderson, L. A., Friedman, L., Osborne-Lawrence, S., Lynch, E., Weissenbach, J., Bowcock, A., King, M.-C. High-absorbance genetic map of the BRCA1 region of chromosome 17q12-q21. Genomics 17: 618-623, 1993. 3. Sosnoski DM, Emanuel BS, Hawkins AL, van Tuinen P, Ledbetter DH, Nussbaum RL, Kaos FT, Schwartz E, Phillips D, Bennett JS, et al. (Aug 1988) . Chromosomal localization of the genes for the vitronectin and fibronectin receptors alpha subunits and for platelet glycoproteins IIb and IIIa.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

87058 MW

Observed Molecular Weight

29 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Integrin beta-3

Subcellular Location

Cell membrane ; Single- pass type I membrane protein . Cell projection, lamellipodium membrane . Cell junction, focal adhesion .

Sequence Similarities

Belongs to the integrin beta chain family.

Protein Name

Integrin beta-3

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.