Anti-Kallistatin/SERPINA4 Antibody Picoband®

About this product

Boster Bio Anti-Kallistatin/SERPINA4 Antibody catalog # PA1638. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1638

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Serpina 4, also called Kallistatin, PI4 or TISSUE KALLIKREIN INHIBITOR, is a protein that in humans is encoded by the SERPINA4 gene. The serpina4 is a member of the serpin superfamily and represents a major inhibitor of human tissue kallikrein in the circulation. The SERPINA4 gene is localized to 14q32.13. The translated amino acid sequence of serpina4 matches the protein sequence and shares 44 to 46% sequence identity with other serpin family members. Kallistatin is a serine proteinase inhibitor (serpin) and a heparin-binding protein. It is localized in vascular smooth muscle cells and endothelial cells of blood vessels, suggesting that it may be involved in the regulation of vascular function. And serpina4 also plays a role in neointima hyperplasia.

Synonyms

Kallistatin; Kallikrein inhibitor; Peptidase inhibitor 4; PI-4; Serpin A4; SERPINA4; KST, PI4

Gene Name

SERPINA4

Gene ID

5267

UniProt

P29622

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Kallistatin.

Clonality

Polyclonal

Tissue Specificity

Expressed by the liver and secreted in plasma.

Applications

IHC, WB

Field of Research

Angiogenesis, Cancer, Cardiovascular, Cell Biology, Invasion/Microenvironment, Protease Inhibitors, Proteolysis/Ubiquitin, Serum Proteins

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Inhibits human amidolytic and kininogenase activities of tissue kallikrein. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein. .

References & Citations

1. Chai, K. X., Chen, L.-M., Chao, J., Chao, L. Kallistatin: a novel human serine proteinase inhibitor: molecular cloning, tissue distribution, and expression in Escherichia coli. J. Biol. Chem. 268: 24498-24505, 1993. 2. Chai, K. X., Ward, D. C., Chao, J., Chao, L. Molecular cloning, sequence analysis, and chromosomal localization of the human protease inhibitor 4 (kallistatin) gene (PI4) . Genomics 23: 370-378, 1994.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

48542 MW

Observed Molecular Weight

91 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Kallistatin

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the serpin family.

Protein Name

Kallistatin

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.