Anti-MCM3 Antibody Picoband®

About this product

Boster Bio Anti-MCM3 Antibody catalog # PA1651. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1651

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

MCM3 (MINICHROMOSOME MAINTENANCE, S. CEREVISIAE, HOMOLOG OF, 3), also called RLFB or P1 PROTEIN, is a protein that in humans is encoded by the MCM3 gene. MCM3 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The MCM3 gene is mapped to 6p12.2. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact ly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.

Synonyms

DNA replication licensing factor MCM3;3.6.4.12; DNA polymerase alpha holoenzyme-associated protein P1; P1-MCM3; RLF subunit beta; p102; MCM3

Gene Name

MCM3

Gene ID

4172

UniProt

P25205

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human MCM3, identical to the related rat and mouse sequences.

Clonality

Polyclonal

Tissue Specificity

Heart, liver, duodenum, blood vessels and kidney.

Applications

Flow Cytometry, IF, IHC, ICC, WB

Field of Research

DNA Synthesis, Epigenetics and Nuclear Signaling

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.

References & Citations

1. Groth, A., Corpet, A., Cook, A. J. L., Roche, D., Bartek, J., Lukas, J., Almouzni, G. Regulation of replication fork progression through histone supply and demand. Science 318: 1928-1931, 2007. 2. Thommes P, Fett R, Schray B, Burkhart R, Barnes M, Kennedy C, Brown NC, Knippers R (Apr 1992) . Properties of the nuclear P1 protein, a mammalian homologue of the yeast Mcm3 replication protein. Nucleic Acids Res 20 (5) : 1069–74. 3. Entrez Gene: MCM3 MCM3 minichromosome maintenance deficient 3 (S. cerevisiae) .

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

90981 MW

Observed Molecular Weight

100-110 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

DNA replication licensing factor MCM3

Subcellular Location

Nucleus.

Sequence Similarities

Belongs to the MCM family.

Protein Name

DNA replication licensing factor MCM3

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.