Anti-Cytoglobin/CYGB Antibody Picoband®

About this product

Boster Bio Anti-Cytoglobin/CYGB Antibody catalog # PA1657. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1657

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cytoglobin (CYGB), also called HGB or STAP, is a ubiquitously expressed hexacoordinate hemoglobin that may facilitate diffusion of oxygen through tissues, scavenge nitric oxide or other reactive oxygen species, or serve a protective function during oxidative stress. The cytoglobin gene is mapped on 17q25.1. The CYGB gene contains 4 exons and spans about 9 kb. Cytoglobin has many elements common to vertebrate globins, including invariant histidine residues, and the amino acids that form the heme pocket share similarity with pentacoordinate myoglobin. In contrast to the high oxygen affinities displayed by most hexacoordinate hemoglobins, the characteristics of CYGB indicate that it can facilitate oxygen transport. Because the oxygen affinity of CYGB is more similar to myoglobin than to neuroglobin, and the oxy form of CYGB resists autooxidation, CYGB is proposed to represent a tissue oxygen reservoir by Sawai et al.

Synonyms

Cytoglobin; Histoglobin; HGb; Stellate cell activation-associated protein; CYGB; STAP

Gene Name

CYGB

Gene ID

114757

UniProt

Q8WWM9

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Cytoglobin, identical to the related rat sequence, different from the related mouse sequence by one amino acid.

Clonality

Polyclonal

Tissue Specificity

Ubiquitously expressed. Highest expression in heart, stomach, bladder and small intestine. .

Applications

WB

Field of Research

Cardiovascular, Serum Proteins

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

May have a protective function during conditions of oxidative stress. May be involved in intracellular oxygen storage or transfer.

References & Citations

1. Asahina, K., Kawada, N., Kristensen, D. B., Nakatani, K., Seki, S., Shiokawa, M., Tateno, C., Obara, M., Yoshizato, K. Characterization of human stellate cell activation-associated protein and its expression in human liver. Biochim. Biophys. Acta 1577: 471-475, 2002. 2. Burmester, T., Ebner, B., Weich, B., Hankeln, T. Cytoglobin: a novel globin type ubiquitously expressed in vertebrate tissues. Molec. Biol. Evol. 19: 416-421, 2002. 3. Geuens, E., Brouns, I., Flamez, D., Dewilde, S., Timmermans, J.-P., Moens, L. A globin in the nucleus! J. Biol. Chem. 278: 30417-30420, 2003.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

21405 MW

Observed Molecular Weight

21 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Cytoglobin

Subcellular Location

Cytoplasm .

Sequence Similarities

Belongs to the APP family.

Protein Name

Cytoglobin

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.