Anti-NOX2/gp91phox/CYBB Antibody Picoband®

About this product

Boster Bio Anti-NOX2/gp91phox/CYBB Antibody catalog # PA1667. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1667

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

NOX2 (NADPH OXIDASE 2), also called CYBB (CYTOCHROME b (-245), BETA SUBUNIT), p91-PHOX or GP91-1, is a human gene encoding a glycoprotein.NOX2 is an essential component of phagocytic NADPH-oxidase, a membrane-bound enzyme complex that generates large quantities of microbicidal superoxide and other oxidants upon activation. It is mapped on Xp11.4. NOX2 is a heterodimer composed of an alpha chain of relative molecular mass 23 kD and a beta chain of 76 to 82 kD. NOX2 assembled on DC phagosomes in a gp91-phox subunit-dependent manner, and that reactive oxygen species were produced in a more sustained manner in immature DC phagosomes than in macrophage phagosomes. As a major player in innate immune responses in neutrophils, NOX2 is also involved in adaptive immunity through its activity in DCs. In heart cells, physiologic stretch rapidly activates reduced-form NOX2 to produce reactive oxygen species (ROS) in a process dependent on microtubules (X-ROS signaling) .

Synonyms

Cytochrome b-245 heavy chain;1.-.-.-; CGD91-phox; Cytochrome b (558) subunit beta; Cytochrome b558 subunit beta; Heme-binding membrane glycoprotein gp91phox; NADPH oxidase 2; Neutrophil cytochrome b 91 kDa polypeptide; Superoxide-generating NADPH oxidase heavy chain subunit; gp91-1; gp91-phox; p22 phagocyte B-cytochrome; CYBB; NOX2

Gene Name

CYBB

Gene ID

1536

UniProt

P04839

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human NOX2, identical to the related rat and mouse sequences.

Clonality

Polyclonal

Tissue Specificity

Detected in neutrophils (at protein level) . .

Applications

IHC, WB

Field of Research

Adaptive Immunity, Cancer, Cancer Metabolism, Immunology, Metabolism, T Cells

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H (+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc.

References & Citations

1. Bionda, C., Li, X. J., van Bruggen, R., Eppink, M., Roos, D., Morel, F., Stasia, M.-J. Functional analysis of two-amino acid substitutions in gp91phox in a patient with X-linked flavocytochrome b (558) -positive chronic granulomatous disease by means of transgenic PLB-985 cells. Hum. Genet. 115: 418-427, 2004. 2. Bolscher, B. G. J. M., de Boer, M., de Klein, A., Weening, R. S., Roos, D. Point mutations in the beta-subunit of cytochrome b (558) leading to X-linked chronic granulomatous disease. Blood 77: 2482-2487, 1991. 3. Entrez Gene: CYBB cytochrome b-245, beta polypeptide (chronic granulomatous disease) .

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

65336 MW

Observed Molecular Weight

65 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Cytochrome b-245 heavy chain

Subcellular Location

Cell membrane; Multi-pass membrane protein.

Sequence Similarities

Contains 1 FAD-binding FR-type domain.

Protein Name

Cytochrome b-245 heavy chain

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.