Anti-Tenascin-R TNR Antibody Picoband®

About this product

Boster Bio Anti-Tenascin-R TNR Antibody catalog # PA1695-1. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1695-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Tenascin-R is a protein that in humans is encoded by the TNR gene. Tenascin-R (TNR) is an extracellular matrix protein expressed primarily in the central nervous system. It is a member of the tenascin (TN) gene family, which includes at least 3 genes in mammals: TNC (or hexabrachion), TNX (TNXB), and TNR. The genes are expressed in distinct tissues at different times during embryonic development and are present in adult tissues. TNR has been detected predominantly in the central nervous system and is localized around motor neurons and on motor axons in the spinal cord, cerebellum, hippocampus, and olfactory bulb. It is suggested that tenascin-R has a role in initiating the detachment of neuroblasts from tangential chains and in initiating radial migration of the cells.

Synonyms

Tenascin-R; TN-R; Janusin; Restrictin; TNR

Gene Name

TNR

Gene ID

7143

UniProt

Q92752

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of mouse TNR, identical to the related rat sequence, and different from the related human sequence by one amino acid.

Clonality

Polyclonal

Tissue Specificity

Brain specific. .

Applications

Flow Cytometry, IHC, WB

Field of Research

Axonal Guidance Proteins, Cell Adhesion Proteins, Cytoskeleton/ECM, Developmental Biology, ECM Proteins, Extracellular Matrix, Growth and Development, Nervous System Development, Neurology Process, Neuroscience, Organogenesis, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Neural extracellular matrix (ECM) protein involved in interactions with different cells and matrix components. These interactions can influence cellular behavior by either evoking a stable adhesion and differentiation, or repulsion and inhibition of neurite growth. Binding to cell surface gangliosides inhibits RGD-dependent integrin-mediated cell adhesion and results in an inhibition of PTK2/FAK1 (FAK) phosphorylation and cell detachment. Binding to membrane surface sulfatides results in a oligodendrocyte adhesion and differentiation. Interaction with CNTN1 induces a repulsion of neurons and an inhibition of neurite outgrowth. Interacts with SCN2B may play a crucial role in clustering and regulation of activity of sodium channels at nodes of Ranvier. TNR-linked chondroitin sulfate glycosaminoglycans are involved in the interaction with FN1 and mediate inhibition of cell adhesion and neurite outgrowth. The highly regulated addition of sulfated carbohydrate structure may modulate the adhesive properties of TNR over the course of development and during synapse maintenance (By similarity) . .

References & Citations

1. Leprini, A., Gherzi, R., Siri, A., Querze, G., Viti, F., Zardi, L.The human tenascin-R gene.J. Biol. Chem. 271: 31251-31254, 1996. 2. Saghatelyan, A., de Chevigny, A., Schachner, M., Lledo, P.-M.Tenascin-R mediates activity-dependent recruitment of neuroblasts in the adult mouse forebrain.Nature Neurosci. 7: 347-356, 2004. 3. Williams, H., Schachner, M., Wang, B., Kenwrick, S.Radiation hybrid mapping of the genes for tenascin-R (TNR), phosducin (PDC), Laminin C1 (LAMC1), and TAX in 1q25-q32.Genomics 46: 165-166, 1997.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

150 kDa

Observed Molecular Weight

180-250 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Tenascin-R

Subcellular Location

Secreted, extracellular space, extracellular matrix.

Sequence Similarities

Belongs to the tenascin family.

Protein Name

Tenascin-R (TN-R)

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.