Anti-CYP11A1 Antibody Picoband®

About this product

Boster Bio Anti-CYP11A1 Antibody catalog # PA1698-1. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1698-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

CYP11A1 (Cytochrome p450, family 11, subfamily A, polypeptide 1), also called CYTOCHROME P450SCC, CYTOCHROME P450C11A1 or CYP11A, is a mitochondrial enzyme associated with the conversion of cholesterol to pregnenolone. CYP11A1 is a member of the cytochrome P450 superfamily of enzymes. The CYP11A1 gene is mapped on 15q24.1. Expression of the CYP11A1 gene may play a role in skin physiology and pathology and that cutaneous proopiomelanocortin activity may be autoregulated by a feedback mechanism involving glucocorticoids synthesized locally by Slominski et al. Using in vitro studies, CYP11A1 catalyzed the side-chain cleavage of 7-dehydrocholesterol to form 7-dehydropregnenolone. In addition, CYP11A1 catalyzed the metabolism of biologically inert vitamin D3, which is formed from 7-dehydrocholesterol, to form 2 hydroxylated products, 20-hydroxyvitamin D3 and 20, 22-dihydroxyvitamin D3. Mutations in the CYP11A1 gene cause congenital adrenal insufficiency with partial or complete 46, XY sex reversal.

Synonyms

Cholesterol side-chain cleavage enzyme, mitochondrial;1.14.15.6; CYPXIA1; Cholesterol desmolase; Cytochrome P450 11A1; Cytochrome P450 (scc) ; Cyp11a1; Cyp11a

Gene Name

CYP11A1

Gene ID

13070

UniProt

Q9QZ82

Host

Rabbit

Reactivity

Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of mouse CYP11A1, different from the related rat sequence by two amino acids.

Clonality

Polyclonal

Applications

WB

Field of Research

Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism of Lipids and Lipoproteins, Mitochondrial Metabolism, Pathways and Processes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone. .

References & Citations

1. al Kandari, H., Katsumata, N., Alexander, S., Rasoul, M. A. Homozygous mutation of P450 side-chain cleavage enzyme gene (CYP11A1) in 46, XY patient with adrenal insufficiency, complete sex reversal, and agenesis of corpus callosum. J. Clin. Endocr. Metab. 91: 2821-2826, 2006. 2. Chung, B.-C., Matteson, K. J., Voutilainen, R., Mohandas, T. K., Miller, W. L. Human cholesterol side-chain cleavage enzyme, P450scc: cDNA cloning, assignment of the gene to chromosome 15, and expression in the placenta. Proc. Nat. Acad. Sci. 83: 8962-8966, 1986. 3. Guryev, O., Carvalho, R. A., Usanov, S., Gilep, A., Estabrook, R. W. A pathway for the metabolism of vitamin D3: unique hydroxylated metabolites formed during catalysis with cytochrome P450scc (CYP11A1) . Proc. Nat. Acad. Sci. 100: 14754-14759, 2003.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

60315 MW

Observed Molecular Weight

60 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Cholesterol side-chain cleavage enzyme, mitochondrial

Subcellular Location

Mitochondrion membrane.

Protein Name

Cholesterol side-chain cleavage enzyme, mitochondrial

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.