- Catalog number
- PA1719
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- SERCA1 (SARCOPLASMIC RETICULUM Ca (2+) -ATPase 1), also called ATP2A1, is an enzyme that in humans is encoded by the ATP2A1 gene. This gene encodes one of the SERCA Ca (2+) -ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. The SERCA1 gene is mapped on 16p11.2. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in muscular excitation and contraction. Zhang et al. (1995) determined that the human ATP2A1 gene is 26 kb long and contains 23 exons, 1 of which can be alternatively spliced.Overexpression of S1T, but not full-length SERCA1, induced ER stress in HeLa cells and amplified ER stress through the PERK (EIF2AK3) -EIF2A -ATF4 -CHOP (DDIT3) pathway. Mutations in this gene cause some autosomal recessive forms of Brody disease, characterized by increasing impairment of muscular relaxation during exercise.
- Synonyms
- Sarcoplasmic/endoplasmic reticulum calcium ATPase 1; SERCA1; SR Ca (2+) -ATPase 1;3.6.3.8; Calcium pump 1; Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform; Endoplasmic reticulum class 1/2 Ca (2+) ATPase; ATP2A1
- Gene Name
- ATP2A1
- Gene ID
- 487
- UniProt
- O14983
- Host
- Rabbit
- Reactivity
- Rat, Mouse, Human
- Cross Reactivity
- No cross-reactivity with other proteins
- Immunogen
- A synthetic peptide corresponding to a sequence in the middle region of human SERCA1 ATPase, identical to the related rat and mouse sequences.
- Clonality
- Polyclonal
- Tissue Specificity
- Skeletal muscle, fast twitch muscle (type II) fibers.
- Applications
- IHC, WB
- Field of Research
- Atherosclerosis, ATPases, Calcium Channels, Calcium Signaling, Cancer, Cardiovascular, Heart Disease, Metabolism, Organelles, Plasma Membrane, Signal Transduction, Signaling Pathway, Subcellular Markers, Tags & Cell Markers
- Purification
- Immunogen affinity purified.
- Concentration
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Function
- Key regulator of striated muscle performance by acting as the major Ca (2+) ATPase responsible for the reuptake of cytosolic Ca (2+) into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.
- References & Citations
- 1. Brandl, C. J., Green, N. M., Korczak, B., MacLennan, D. H. Two Ca (2+) ATPase genes: homologies and mechanistic implications of deduced amino acid sequences. Cell 44: 597-607, 1986. 2. Karpati, G., Charuk, J., Carpenter, S., Jablecki, C., Holland, P. Myopathy caused by a deficiency of Ca (2+) -adenosine triphosphatase in sarcoplasmic reticulum (Brody's disease) . Ann. Neurol. 20: 38-49, 1986. 3. Zhang, Y., Fujii, J., Phillips, M. S., Chen, H.-S., Karpati, G., Yee, W.-C., Schrank, B., Cornblath, D. R., Boyland, K. B., MacLennan, D. H. Characterization of cDNA and genomic DNA encoding SERCA1, the Ca (2+) -ATPase of human fast-twitch skeletal muscle sarcoplasmic reticulum, and its elimination as a candidate gene for Brody disease. Gemomics 30: 415-424, 1995.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 110252 MW
- Observed Molecular Weight
- 29 kDa
- Specificity
- No cross reactivity with other proteins.
- Gene Name Synonym
- Sarcoplasmic/endoplasmic reticulum calcium ATPase 1
- Subcellular Location
- Endoplasmic reticulum membrane; Multi-pass membrane protein. Sarcoplasmic reticulum membrane; Multi-pass membrane protein.
- Protein Name
- Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 (SERCA1/SR Ca (2+) -ATPase 1)
- Isotype
- Rabbit IgG
- Contents
- Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
