Anti-Glucose Transporter GLUT4/SLC2A4 Antibody Picoband®

About this product

Boster Bio Anti-Glucose Transporter GLUT4/SLC2A4 Antibody catalog # PA1722. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1722

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Facilitated glucose transport by mammalian cells is not a property of a single protein but an activity associated with a family of structurally related proteins. Glucose transporter 4 is a insulin-responsive glucose transporter. It belongs to solute carrier family 2, member 1. Insulin alters the subcellular localization of GLUT4 vesicles in human muscle, and that this effect is impaired equally in insulin-resistant subjects with and without diabetes. A similar pattern of defects cause insulin resistance in human adipocytes.Human insulin resistance involves a defect in GLUT4 traffic and targeting leading to accumulation in a dense membrane compartment from which insulin is unable to recruit GLUT4 to the cell surface.

Synonyms

Solute carrier family 2, facilitated glucose transporter member 4; Glucose transporter type 4, insulin-responsive; GLUT-4; SLC2A4; GLUT4

Gene Name

SLC2A4

Gene ID

6517

UniProt

P14672

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human GLUT4, identical to the related rat and mouse sequences.

Clonality

Polyclonal

Tissue Specificity

Skeletal and cardiac muscles; brown and white fat.

Applications

IHC, WB

Field of Research

Cardiovascular, Energy Metabolism, Energy Transfer Pathways, Epigenetics and Nuclear Signaling, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction, Transcription, Transcription Factors

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Insulin-regulated facilitative glucose transporter.

References & Citations

1. Birnbaum, M. J. : Identification of a novel gene encoding an insulin-responsive glucose transporter protein. Cell 57: 305-315, 1989. 2. Bell, G. I.; Kayano, T.; Buse, J. B.; Burant, C. F.; Takeda, J.; Lin, D.; Fukumoto, H.; Seino, S. : Molecular biology of mammalian glucose transporters. Diabetes Care 13: 198-208, 1990. 3. Garvey, W. T.; Maianu, L.; Zhu, J.-H.; Brechtel-Hook, G.; Wallace, P.; Baron, A. D. : Evidence for defects in the trafficking and translocation of GLUT4 glucose transporters in skeletal muscle as a cause of human insulin resistance. J. Clin. Invest. 101: 2377-2386, 1998.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

54787 MW

Observed Molecular Weight

55 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Solute carrier family 2, facilitated glucose transporter member 4

Subcellular Location

Cell membrane ; Multi-pass membrane protein . Endomembrane system ; Multi-pass membrane protein . Cytoplasm, perinuclear region . Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization motif is critical for intracellular sequestration.

Sequence Similarities

Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.

Protein Name

Solute carrier family 2, facilitated glucose transporter member 4

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.