Anti-PAK6 Antibody Picoband®

About this product

Boster Bio Anti-PAK6 Antibody catalog # PA1729. Tested in Flow Cytometry, ICC/IF, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1729

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Serine/threonine-protein kinase PAK 6 (p21 Protein-activated Kinase 6) is an enzyme that in humans is encoded by the PAK6 gene. The PAK6 gene is mapped to chromosome 15q15. based on an alignment of the PAK6 sequence with the genomic sequence. This gene encodes a protein that shares a high degree of sequence similarity with p21-activated kinase (PAK) family members. The proteins of this family are Rac/Cdc42-associated Ste20-like Ser/Thr protein kinases, characterized by a highly conserved amino-terminal Cdc42/Rac interactive binding (CRIB) domain and a carboxyl-terminal kinase domain. PAK kinases are implicated in the regulation of a number of cellular processes, including cytoskeleton rearrangement, apoptosis and the MAP kinase signaling pathway. The protein encoded by this gene was found to interact with androgen receptor (AR), which is a steroid hormone-dependent transcription factor that is important for male sexual differentiation and development. The p21-activated protein kinase 6 gene was found to be highly expressed in testis and prostate tissues and the encoded protein was shown to cotranslocate into the nucleus with AR in response to androgen.

Synonyms

Serine/threonine-protein kinase PAK 6;2.7.11.1; PAK-5; p21-activated kinase 6; PAK-6; PAK6; PAK5

Gene Name

PAK6

Gene ID

106821730

UniProt

Q9NQU5

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human PAK6, identical to the related rat and mouse sequences.

Clonality

Polyclonal

Tissue Specificity

Selectively expressed in brain and testis, with lower levels in multiple tissues including prostate and breast. .

Applications

Flow Cytometry, ICC/IF, WB

Field of Research

Protein Phosphorylation, Ser/Thr Kinases, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Serine/threonine protein kinase that plays a role in the regulation of gene transcription. The kinase activity is induced by various effectors including AR or MAP2K6/MAPKK6. Phosphorylates the DNA-binding domain of androgen receptor/AR and thereby inhibits AR-mediated transcription. Inhibits also ESR1-mediated transcription. May play a role in cytoskeleton regulation by interacting with IQGAP1. May protect cells from apoptosis through phosphorylation of BAD. .

References & Citations

1. Gross, M. B. Personal Communication. Baltimore, Md. 5/10/2011. 2. Yang, F., Li, X., Sharma, M., Zarnegar, M., Lim, B., Sun, Z. Androgen receptor specifically interacts with a novel p21-activated kinase, PAK6. J. Biol. Chem. 276: 15345-15353, 2001.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

75 kDa

Observed Molecular Weight

70-75 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Serine/threonine-protein kinase PAK 6

Subcellular Location

Cytoplasm. Nucleus. Cotranslocates into nucleus with AR in response to androgen induction.

Sequence Similarities

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

Protein Name

Serine/threonine-protein kinase PAK 6

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.