- Catalog number
- PA1743
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- CD2 (cluster of differentiation 2) is a cell adhesion molecule found on the surface of T cells and natural killer (NK) cells. It has also been called T-cell surface antigen T11/Leu-5, LFA-2, LFA-3 receptor, erythrocyte receptor and rosette receptor. Monoclonal antibodies ed against CD2 inhibit the formation of rosettes with sheep erythrocytes, indicating that CD2 is the erythrocyte receptor or is closely associated with it. It is one of the earliest T-cell markers, being present on more than 95% of thymocytes; it is also found on some natural killer cells but not on B lymphocytes. Due to its structural characteristics, CD2 is a member of the immunoglobulin superfamily; it possesses two immunoglobulin-like domains in its extracellular portionThe localization of CD2 to 1p13 was established by in situ hybridization. By Southern blotting of DNA from a panel of somatic cell hybrids, Clayton et al. (1988) assigned the CD2 gene to human chromosome 1 and murine chromosome 3.CD2 interacts with other adhesion molecules, such as lymphocyte function-associated antigen-3 (LFA-3/CD58) in humans, or CD48 in rodents, which are expressed on the surfaces of other cells. With the use of transgenic mice, such an LCR was identified within the 3-prime flanking region of the human CD2 gene.
- Synonyms
- T-cell surface antigen CD2; LFA-2; LFA-3 receptor; Lymphocyte antigen 37; Ly-37; T-cell surface antigen T11/Leu-5; CD2; Cd2; Ly-37
- Gene Name
- CD2
- Gene ID
- 914
- UniProt
- P08920
- Host
- Rabbit
- Reactivity
- Mouse, Rat
- Cross Reactivity
- No cross-reactivity with other proteins
- Immunogen
- A synthetic peptide corresponding to a sequence in the middle region of mouse CD2, different from the related rat sequence by two amino acids.
- Clonality
- Polyclonal
- Applications
- WB
- Field of Research
- Adaptive Immunity, Hematopoietic Progenitors, Immunology, Lymphoid, Myeloid, Stem Cells, T Cells, T Lymphocytic Lineage
- Purification
- Immunogen affinity purified.
- Concentration
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Function
- CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T- cells, the cytoplasmic domain is implicated in the signaling function.
- References & Citations
- 1. Brown, M. H., Gorman, P. A., Sewell, W. A., Spurr, N. K., Sheer, D., Crumpton, M. J. The gene coding for the human T-lymphocyte CD2 antigen is located on chromosome 1p. Hum. Genet. 76: 191-195, 1987. 2. Clayton, L. K., Ramachandran, H., Pravtcheva, D., Chen, Y.-F., Diamond, D. J., Ruddle, F. H., Reinherz, E. L. The gene for T11 (CD2) maps to chromosome 1 in humans and to chromosome 3 in mice. J. Immun. 140: 3617-3621, 1988. 3. Kingsmore, S. F., Watson, M. L., Moseley, W. S., Seldin, M. F. Physical linkage of genes encoding the lymphocyte adhesion molecules CD2 and its ligand LFA-3. Immunogenetics 30: 123-125, 1989.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 38415 MW
- Observed Molecular Weight
- 65 kDa
- Specificity
- No cross reactivity with other proteins.
- Gene Name Synonym
- T-cell surface antigen CD2
- Subcellular Location
- Membrane; Single-pass type I membrane protein.
- Sequence Similarities
- Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
- Protein Name
- T-cell surface antigen CD2
- Isotype
- Rabbit IgG
- Contents
- Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
