Anti-TRPC6 Antibody Picoband®

About this product

Boster Bio Anti-TRPC6 Antibody catalog # PA1754. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1754

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Transient receptor potential cation channel, subfamily C, member 6, also known as TRPC6, is a human gene encoding a protein of the same name. The protein encoded by this gene forms a receptor-activated calcium channel in the cell membrane. The channel is activated by diacylglycerol and is thought to be under the control of a phosphatidylinositol second messenger system. Activation of this channel occurs independently of protein kinase C and is not triggered by low levels of intracellular calcium. Defects in this gene are a cause of focal segmental glomerulosclerosis 2 (FSGS2) .

Synonyms

Short transient receptor potential channel 6; TrpC6; Transient receptor protein 6; TRP-6; TRPC6; TRP6

Gene Name

TRPC6

Gene ID

7225

UniProt

Q9Y210

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human TRPC6, different from the related rat and mouse sequences by three amino acids.

Clonality

Polyclonal

Tissue Specificity

Expressed primarily in placenta, lung, spleen, ovary and small intestine. Expressed in podocytes and is a component of the glomerular slit diaphragm. .

Applications

IHC, WB

Field of Research

Calcium Channels, Calcium Signaling, Signal Transduction, Signaling Pathway

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C. Seems not to be activated by intracellular calcium store depletion.

References & Citations

1. D'Esposito, M., Strazzullo, M., Cuccurese, M., Spalluto, C., Rocchi, M., D'Urso, M., Ciccodicola, A. Identification and assignment of the human transient receptor potential channel 6 gene TRPC6 to chromosome 11q21-q22. Cytogenet. Cell Genet. 83: 46-47, 1998. 2. Hofmann, T., Obukhov, A. G., Schaefer, M., Harteneck, C., Gudermann, T., Schultz, G. activation of human TRPC6 and TRPC3 channels by diacylglycerol. Nature 397: 259-263, 1999. 3. Kwon, Y., Hofmann, T., Montell, C. Integration of phosphoinositide- and calmodulin-mediated regulation of TRPC6. Molec. Cell 25: 491-503, 2007.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

106326 MW

Observed Molecular Weight

106 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Short transient receptor potential channel 6

Subcellular Location

Membrane ; Multi-pass membrane protein .

Sequence Similarities

Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC6 sub-subfamily.

Protein Name

Short transient receptor potential channel 6 (TrpC6)

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.