Anti-DCI/ECI1 Antibody Picoband®

About this product

Boster Bio Anti-DCI/ECI1 Antibody catalog # PA1763. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1763

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

ECI1/DCI (Dodecenoyl-CoA Delta Isomerase), also known as 3,2-trans-enoyl-CoA isomerase, is an enzyme that catalyzes the conversion of cis-or trans-double bonds of fatty acids at gamma-carbon (position 3) to trans double bonds at beta-carbon (position 2) . It plays a particularly important role in the metabolism of unsaturated fatty acids. All classes of enoyl-CoA isomerases belong to a family of enzymes, the hydratase/isomerase or crotonase superfamily, and when examined with x-ray crystallography, exhibit a common structural feature of the family, the N-terminal core with a spiral fold composed of four turns, each turn consisting of two beta-sheets and one alpha-helix. Dodecenoyl-CoA Delta Isomerase is involved in the beta-oxidation, one of the most frequently used pathways in fatty acid degradation, of unsaturated fatty acids with double bonds at odd-numbered carbon positions. It does so by shifting the position of the double bonds in the acyl-CoA intermediates and converting 3-cis or trans-enoyl-CoA to 2-trans-enoyl-CoA.

Synonyms

Enoyl-CoA delta isomerase 1, mitochondrial;5.3.3.8;3,2-trans-enoyl-CoA isomerase; Delta (3), Delta (2) -enoyl-CoA isomerase; D3, D2-enoyl-CoA isomerase; Dodecenoyl-CoA isomerase; ECI1; DCI

Gene Name

ECI1

Gene ID

1632

UniProt

P42126

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human DCI, different from the related mouse sequence by two amino acids and from the related rat sequence by three amino acids.

Clonality

Polyclonal

Applications

Flow Cytometry, IHC, WB

Field of Research

Cancer, Cancer Metabolism, Cardiovascular, Fatty Acid Oxidation, Fatty Acids, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism of Lipids and Lipoproteins, Mitochondria, Mitochondrial, Mitochondrial Markers, Mitochondrial Metabolism, Organelles, Pathways and Processes, Redox Metabolism, Signal Transduction, Subcellular Markers, Tags & Cell Markers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species.

References & Citations

1. Janssen, U., Fink, T., Lichter, P., Stoffel, W.Human mitochondrial 3,2-trans-enoyl-CoA isomerase (DCI) : gene structure and localization to chromosome 16p13.3.Genomics 23: 223-228, 1994.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

33 kDa

Observed Molecular Weight

28 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Enoyl-CoA delta isomerase 1, mitochondrial

Subcellular Location

Mitochondrion matrix.

Protein Name

Enoyl-CoA delta isomerase 1, mitochondrial

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.