- Catalog number
- PA1764
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- FMO3 (Flavin-containing Monooxygenase 3) is an enzyme that in humans is encoded by the FMO3 gene. The mammalian flavin-containing monooxygenases (FMO) represent a multigene family whose gene products are localized in the endoplasmic reticulum of many tissues. The FMO3 gene contains 1 noncoding and 8 coding exons. The FMO3 gene is mapped on 1q24.3. Using quantitative RNase protection assays, FMO3 is present in low abundance in fetal liver and lung and in adult kidney and lung, and in much greater abundance in adult liver. By Western blot analysis of human liver microsomal samples ranging from 8 weeks gestation to 18 years of age, FMO1 is the major fetal isoform and FMO3 is the major adult isoform. FMO3 was expressed at intermediate levels until 11 years of age when a gender-independent increase in FMO3 expression was observed during puberty. Sufferers of trimethylaminuria may display a reduced ability to metabolize substrates for FMO3 such as nicotine. FMO3 metabolizes a number of drugs, including amphetamine, clozapine, deprenyl, metamphetamine, tamoxifen, ethionamide, thiacetazone, and sulindac sulfide.
- Synonyms
- Dimethylaniline monooxygenase [N-oxide-forming] 3;1.14.13.8; Dimethylaniline oxidase 3; FMO II; FMO form 2; Hepatic flavin-containing monooxygenase 3; FMO 3; Trimethylamine monooxygenase;1.14.13.148; FMO3
- Gene Name
- FMO3
- Gene ID
- 2328
- UniProt
- P31513
- Host
- Rabbit
- Reactivity
- Rat, Mouse, Human
- Cross Reactivity
- No cross-reactivity with other proteins
- Immunogen
- A synthetic peptide corresponding to a sequence at the N-terminus of human FMO3, different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino acids.
- Clonality
- Polyclonal
- Tissue Specificity
- Liver.
- Applications
- WB
- Field of Research
- Cancer, Drug Metabolism, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction
- Purification
- Immunogen affinity purified.
- Concentration
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Function
- Involved in the oxidative metabolism of a variety of xenobiotics such as drugs and pesticides. It N-oxygenates primary aliphatic alkylamines as well as secondary and tertiary amines. Plays an important role in the metabolism of trimethylamine (TMA), via the production of TMA N-oxide (TMAO) . Is also able to perform S-oxidation when acting on sulfide compounds (PubMed:9224773) . .
- References & Citations
- 1. Akerman, B. R., Chow, L., Forrest, S., Youil, R., Cashman, J., Treacy, E. P. Mutations in the flavin-containing monoxygenase (sic) form 3 (FMO3) gene cause trimethylaminuria, fish odour syndrome. (Abstract) Am. J. Hum. Genet. 61 (suppl.) : A53 only, 1997. 2. Cashman, J. R., Zhang, J. Interindividual differences of human flavin-containing monooxygenase 3: genetic polymorphisms and functional variation. Drug Metab. Dispos. 30: 1043-1052, 2002. 3. Dolphin, C. T., Janmohamed, A., Smith, R. L., Shephard, E. A., Phillips, I. R. Missense mutation in flavin-containing mono-oxygenase 3 gene, FMO3, underlies fish-odour syndrome. Nature Genet. 17: 491-494, 1997.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 60033 MW
- Observed Molecular Weight
- 33 kDa
- Specificity
- No cross reactivity with other proteins.
- Gene Name Synonym
- Dimethylaniline monooxygenase [N-oxide-forming] 3
- Subcellular Location
- Microsome membrane. Endoplasmic reticulum membrane.
- Sequence Similarities
- Belongs to the FMO family.
- Protein Name
- Dimethylaniline monooxygenase [N-oxide-forming] 3
- Isotype
- Rabbit IgG
- Contents
- Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
