Anti-SNAP23 Antibody Picoband®

About this product

Boster Bio Anti-SNAP23 Antibody catalog # PA1774. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1774

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

SNAP23 (Synaptosomal-Associated Protein, 23-KD), also called SNAP23A, is a protein that in humans is encoded by the SNAP23 gene. The SNAP23 gene has 8 exons, with the initiation codon located in exon 2. The SNAP23 gene is mapped on 15q15.1-q15.2. The SNAP23 cDNA encodes a 211-amino acid polypeptide with a predicted mass of 23 kD. Its amino acid sequence is 59% identical to that of SNAP25. Northern blot analysis revealed that SNAP23 is ubiquitously expressed. SNAP23 is able to bind to multiple syntaxins as well as to multiple vesicle-associated membrane proteins. After relocation, SNAP23 is required for exocytosis, implying a crucial role in promoting membrane fusion. TIVAMP-containing vesicles were concentrated in the apical domain of epithelial cells. STX3A and SNAP23 were codistributed at the apical plasma membrane, where they formed N-ethyl maleimide-dependent SNARE complexes with TIVAMP and cellubrevin. SNAP23 is structurally and functionally similar to SNAP25 and binds tightly to multiple syntaxins and synaptobrevins/VAMPs. It is an essential component of the high affinity receptor for the general membrane fusion machinery and is an important regulator of transport vesicle docking and fusion.

Synonyms

Synaptosomal-associated protein 23; SNAP-23; Vesicle-membrane fusion protein SNAP-23; SNAP23

Gene Name

SNAP23

Gene ID

8773

UniProt

O00161

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human SNAP23, different from the related rat and mouse sequences by three amino acids.

Clonality

Polyclonal

Tissue Specificity

Ubiquitous. Highest levels where found in placenta.

Applications

Flow Cytometry, IF, IHC, IHC-F, ICC, WB

Field of Research

Cell Type Marker, Neuron Marker, Neuroscience, Neurotransmission, Secretory Vesicles, Synapse Marker

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Essential component of the high affinity receptor for the general membrane fusion machinery and an important regulator of transport vesicle docking and fusion.

References & Citations

1. Guo, Z., Turner, C., Castle, D. Relocation of the t-SNARE SNAP-23 from lamellipodia-like cell surface projections regulates compound exocytosis in mast cells. Cell 94: 537-548, 1998. 2. Lazo, P. A., Nadal, M., Ferrer, M., Area, E., Hernandez-Torres, J., Nabokina, S. M., Mollinedo, F., Estivill, X. Genomic organization, chromosomal localization, alternative splicing, and isoforms of the human synaptosome-associated protein-23 gene implicated in vesicle-membrane fusion processes. Hum. Genet. 108: 211-215, 2001. 3. Mollinedo, F., Lazo, P. A. Identification of two isoforms of the vesicle-membrane fusion protein SNAP-23 in human neutrophils and HL-60 cells. Biochem. Biophys. Res. Commun. 231: 808-812, 1997.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

23 kDa

Observed Molecular Weight

23 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Synaptosomal-associated protein 23

Subcellular Location

Cell membrane; Peripheral membrane protein. Cell membrane; Lipid-anchor. Cell junction, synapse, synaptosome. Mainly localized to the plasma membrane.

Sequence Similarities

Belongs to the SNAP-25 family.

Protein Name

Synaptosomal-associated protein 23 (SNAP-23)

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.