Anti-Apoptosis repressor with CARD/NOL3 Antibody Picoband®

About this product

Boster Bio Anti-Apoptosis repressor with CARD/NOL3 Antibody catalog # PA1793. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1793

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

NOL3 (Nucleolar protein 3), also known as ARC, NOP30, CARD2 and MYP, is a protein that in humans is encoded by the NOL3 gene. NOL3 has been shown to interact with SFRS9 and Caspase 8. By genomic sequence analysis, Stoss et al. (1999) determined that the NOL3 gene, which encodes NOP30 and MYP and which they called NOP, is composed of 4 exons. The alternative 5-prime splice site that generates the 2 isoforms is located in exon 2. It is reported that expression of the ARC cDNA encoding the smaller transcript inhibited apoptosis in a dose-dependent manner when coexpressed with CASP8 but not when coexpressed with CASP9. ARC also inhibited apoptosis induced by stimulation of CD95/FAS, tumor necrosis factor receptor-1, and TRAMP/death receptor-3. Enzymatic analysis showed that ARC inhibits the enzymatic activity of CASP8. Immunoprecipitation and immunoblot analysis indicated that ARC interacts with CASP2 and CASP8 through its N-terminal death effector domain but does not interact with CASP1, CASP3, or CASP9.

Synonyms

Nucleolar protein 3 ; Apoptosis repressor with CARD ; Muscle-enriched cytoplasmic protein ; Myp ; Nucleolar protein of 30 kDa ; Nop30 ; NOL3 ; ARC , NOP

Gene Name

NOL3

Gene ID

8996

UniProt

O60936

Host

Rabbit

Reactivity

Human, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Apoptosis repressor with CARD, identical to the related rat sequence, and different from the related mouse sequence by one amino acid.

Clonality

Polyclonal

Tissue Specificity

Highly expressed in heart and skeletal muscle. Detected at low levels in placenta, liver, kidney and pancreas.

Applications

WB

Field of Research

Apoptosis, Caspases, Cell Biology, Intracellular

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Isoform 1: May be involved in RNA splicing. .

References & Citations

1. Abmayr, S., Crawford, R. W., Chamberlain, J. S.Characterization of ARC, apoptosis repressor interacting with CARD, in normal and dystrophin-deficient skeletal muscle.Hum. Molec. Genet. 13: 213-221, 2004. 2. Koseki, T., Inohara, N., Chen, S., Nunez, G.ARC, an inhibitor of apoptosis expressed in skeletal muscle and heart that interacts selectively with caspases.Proc. Nat. Acad. Sci. 95: 5156-5160, 1998. 3. Stoss, O., Schwaiger, F.-W., Cooper, T. A., Stamm, S.Alternative splicing determines the intracellular localization of the novel nuclear protein Nop30 and its interaction with the splicing factor SRp30c.J. Biol. Chem. 274: 10951-10962, 1999.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

23 kDa

Observed Molecular Weight

27 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Nucleolar protein 3

Subcellular Location

Isoform 1: Nucleus, nucleolus . The SR-rich C-terminus mediates nuclear localization. .

Sequence Similarities

Contains 1 CARD domain.

Protein Name

Nucleolar protein 3

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg Thimerosal, 0.01mg NaN3.