Anti-14-3-3 sigma/SFN Antibody Picoband®

About this product

Boster Bio Anti-14-3-3 sigma/SFN Antibody catalog # PA1797. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1797

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Stratifin (SFN), also known as 14-3-3 protein sigma, is strongly induced by gamma irradiation and other DNA-damaging agents. The induction of 14-3-3-sigma is mediated by a p53 -responsive element located 1.8 kb upstream of its transcription start site. Leffers et al. (1993) obtained peptide sequence and subsequently cloned a T-cell cDNA of the 14-3-3 family of conserved proteins. The protein, called stratifin, was shown to be diffusely distributed in the cytoplasm and was present in cultured epithelial cells. It was most abundant in tissues enriched in stratified keratinizing epithelium.

Synonyms

14-3-3 protein sigma; Epithelial cell marker protein 1; Stratifin; SFN; HME1

Gene Name

SFN

Gene ID

2810

UniProt

P31947

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human 14-3-3 sigma, identical to the related rat and mouse sequences.

Clonality

Polyclonal

Tissue Specificity

Present mainly in tissues enriched in stratified squamous keratinizing epithelium.

Applications

Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Adapters, Cell Type Marker, Cytoplasmic, Neural Signal Transduction, Neurology Process, Neuron Marker, Neuroscience, Signal Transduction, Signaling Pathways, Stem Cells, Synapse Marker

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53. .

References & Citations

1. Leffers, H., Madsen, P., Rasmussen, H. H., Honore, B., Andersen, A. H., Walbum, E., Vandekerckhove, J., Celis, J. E.Molecular cloning and expression of the transformation sensitive epithelial marker stratifin: a member of a protein family that has been involved in the protein kinase C signalling pathway.J. Molec. Biol. 231: 982-998, 1993. 2. Urano, T., Saito, T., Tsukui, T., Fujita, M., Hosoi, T., Muramatsu, M., Ouchi, Y., Inoue, S. Efp targets 14-3-3-sigma for proteolysis and promotes breast tumour growth.Nature 417: 871-875, 2002. 3. Wilker, E. W., van Vugt, M. A. T. M., Artim, S. A., Huang, P. H., Petersen, C. P., Reinhardt, H. C., Feng, Y., Sharp, P. A., Sonenberg, N., White, F. M., Yaffe, M. B.14-3-3-sigma controls mitotic translation to facilitate cytokinesis.Nature 446: 329-332, 2007.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

27774 MW

Observed Molecular Weight

28 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

14-3-3 protein sigma

Subcellular Location

Cytoplasm. Nucleus . Secreted. May be secreted by a non-classical secretory pathway.

Protein Name

14-3-3 protein sigma

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.