Anti-Crk-like protein CrkL Antibody Picoband®

About this product

Boster Bio Anti-Crk-like protein CrkL Antibody catalog # PA1808. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1808

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

CRKL (v-crk sarcoma virus CT10 oncogene homolog (avian) -like) also called ONCOGENE  CRKL, encodes a 303-amino acid polypeptide with a predicted molecular mass of 36 kD. While  CRKL  is not the human homolog of CRK, it is similar to protein-tyrosine kinases with SH2 and SH3 (src homology) domains. By fluorescence in situ hybridization,   the  CRKL  gene is localized to chromosome 22q11, centromeric of the chronic myelogenous leukemia breakpoint region. Senechal et al. (1996)   showed that  CRKL  becomes phosphorylated when overexpressed, activates Ras-dependent and JNK pathways, and transforms fibroblasts. The authors also found  CRKL  to be a substrate for the BCR-ABL tyrosine kinase, leading them to conclude thatCRKL  is a tyrosine kinase and an oncogene. Crkl  was required for normal cellular responses to Fgf8, including survival and migration, Erk activation, and target gene expression.

Synonyms

Crk-like protein; CRKL

Gene Name

CRKL

Gene ID

1399

UniProt

P46109

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human CrkL, different from the related mouse and rat sequences by one amino acid.

Clonality

Polyclonal

Applications

WB, Flow Cytometry

Field of Research

Oncoproteins/Suppressors, Proto-Oncogenes, Signal Transduction, Transcription

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

May mediate the transduction of intracellular signals.

References & Citations

1. Guris, D. L., Duester, G., Papaioannou, V. E., Imamoto, A. Dose-dependent interaction of Tbx1 and Crkl and locally aberrant RA signaling in a model of del22q11 syndrome. Dev. Cell 10: 81-92, 2006. 2. Moon, A. M., Guris, D. L., Seo, J., Li, L., Hammond, J., Talbot, A., Imamoto, A. Crkl deficiency disrupts Fgf8 signaling in a mouse model of 22q11 deletion syndromes. Dev. Cell 10: 71-80, 2006. 3. Senechal, K., Halpern, J., Sawyers, C. L. The CRKL adaptor protein transforms fibroblasts and functions in transformation by the BCR-ABL oncogene. J. Biol. Chem. 38: 23255-23261, 1996.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

33777 MW

Observed Molecular Weight

39 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Crk-like protein

Subcellular Location

Nucleus. Cytoplasm.

Sequence Similarities

Belongs to the CRK family.

Protein Name

Crk-like protein

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.