Anti-Hyaluronidase-2 HYAL2 Antibody Picoband®

About this product

Boster Bio Anti-Hyaluronidase-2 HYAL2 Antibody catalog # PA1817. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1817

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

HYAL2 (hyaluronoglucosaminidase 2) also known as hyaluronidase 2, LuCa-2, is an  enzyme  that in humans is encoded by the  HYAL2  gene. HYAL2  cDNAs encode a preprotein with an N-terminal signal peptide.  Northern blot analysis indicated that  HYAL2  was expressed in all human tissues tested except adult brain, and Western blot analysis detected  Hyal2protein in all mouse tissues examined except adult brain. The HYAL2 gene is mapped on 3p21.31. HYAL2  is a glycosylphosphatidylinositol (GPI) -anchored protein on the cell surface and serves as a receptor for entry into the cell of the jaagsiekte sheep retrovirus (JSRV) . The findings of  Rai et al. (2001)   that  HYAL2  is a GPI-anchored protein on the cell surface showed that GFP would likely be cleaved from  HYAL2  during GPI addition, leaving  HYAL2  on the cell surface and resulting in GFP transit to the lysosome for degradation.

Synonyms

Hyaluronidase-2; Hyal-2;3.2.1.35; Hyaluronoglucosaminidase-2; Lung carcinoma protein 2; LuCa-2; HYAL2; LUCA2

Gene Name

HYAL2

Gene ID

8692

UniProt

Q12891

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human HYAL2, different from the related mouse and rat sequences by three amino acids.

Clonality

Polyclonal

Tissue Specificity

Widely expressed. No expression detected in adult brain. .

Applications

WB

Field of Research

Cancer, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product which is further hydrolyzed by sperm hyaluronidase to give small oligosaccharides. Displays very low levels of activity. Associates with and negatively regulates MST1R. .

References & Citations

1. Lepperdinger, G., Strobl, B., Kreil, G. HYAL2, a human gene expressed in many cells, encodes a lysosomal hyaluronidase with a novel type of specificity. J. Biol. Chem. 273: 22466-22470, 1998. 2. Rai, S. K., Duh, F.-M., Vigdorovich, V., Danilkovitch-Miagkova, A., Lerman, M. I., Miller, A. D. Candidate tumor suppressor HYAL2 is a glycosylphosphatidylinositol (GPI) -anchored cell-surface receptor for jaagsiekte sheep retrovirus, the envelope protein of which mediates oncogenic transformation. Proc. Nat. Acad. Sci. 98: 4443-4448, 2001. 3. Strobl, B., Wechselberger, C., Beier, D. R., Lepperdinger, G. Structural organization and chromosomal localization of Hyal2, a gene encoding a lysosomal hyaluronidase. Genomics 53: 214-219, 1998.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

53860 MW

Observed Molecular Weight

54 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Hyaluronidase-2

Subcellular Location

Cell membrane ; Lipid-anchor, GPI-anchor .

Sequence Similarities

Belongs to the glycosyl hydrolase 56 family.

Protein Name

Hyaluronidase-2 (Hyal-2)

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.