Anti-Interferon regulatory factor 2 IRF2 Antibody Picoband®

About this product

Boster Bio Anti-Interferon regulatory factor 2 IRF2 Antibody catalog # PA1818. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1818

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

IRF2 (interferon regulatory factor 2) is   a member of the interferon regulatory transcription factor (IRF) family. The IRF2 gene is mapped on 4q35.1. When the  IRF2  gene was overexpressed in NIH 3T3 cells, the cells became transformed and displayed enhanced tumorigenicity in nude mice. One IRF binding site was found within the  IRF2  promoter, and expression of the  IRF2  gene was affected by both transient and stable IRF1 expression. IRF2 competitively inhibits the  IRF1-mediated transcriptional activation of interferons alpha and beta, and presumably other genes that employ IRF1 for transcription activation. However, IRF2 also functions as a transcriptional activator of  histone H4. Irf2  was required to prevent NK-cell apoptosis and keep immature NK cells alive, thus promoting NK-cell maturation and their supply to peripheral blood.

Synonyms

Interferon regulatory factor 2; IRF-2; IRF2

Gene Name

IRF2

Gene ID

3660

UniProt

P14316

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human IRF2, identical to the related rat sequence and different from the related mouse sequence by one amino acid.

Clonality

Polyclonal

Tissue Specificity

Expressed throughout the epithelium of the colon. Also expressed in lamina propria. .

Applications

WB

Field of Research

Cytokines, Domain Families, Epigenetics and Nuclear Signaling, Hlh/Leucine Zipper, Immunology, Innate Immunity, Interferons, Pol II Transcription, Polymerase Associated Factors, Transcription, Transcription Factors

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS) ) and represses those genes. Also acts as an activator for several genes including H4 and IL7. Constitutively binds to the ISRE promoter to activate IL7. Involved in cell cycle regulation through binding the site II (HiNF-M) promoter region of H4 and activating transcription during cell growth. Antagonizes IRF1 transcriptional activation. .

References & Citations

1. Harada, H., Takahashi, E.-I., Itoh, S., Harada, K., Hori, T.-A., Taniguchi, T. Structure and regulation of the human interferon regulatory factor 1 (IRF-1) and IRF-2 genes: implications for a gene network in the interferon system. Molec. Cell. Biol. 14: 1500-1509, 1994. 2. Nishio, Y., Noguchi, E., Ito, S., Ichikawa, E., Umebayashi, Y., Otsuka, F., Arinami, T. Mutation and association analysis of the interferon regulatory factor 2 gene (IRF2) with atopic dermatitis. J. Hum. Genet. 46: 664-667, 2001. 3. Taki, S., Nakajima, S., Ichikawa, E., Saito, T., Hida, S. IFN regulatory factor-2 deficiency revealed a novel checkpoint critical for the generation of peripheral NK cells. J. Immun. 174: 6005-6012, 2005.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

39354 MW

Observed Molecular Weight

39 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Interferon regulatory factor 2

Subcellular Location

Nucleus.

Sequence Similarities

Belongs to the IRF family.

Protein Name

Interferon regulatory factor 2 (IRF-2)

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.