Anti-Kallikrein 10/KLK10 Antibody Picoband®

About this product

Boster Bio Anti-Kallikrein 10/KLK10 Antibody catalog # PA1821. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1821

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

KLK10 (kallikrein-related peptidase 10) also known as kallikrein 10, PRSSL1 or NES1, belongs to the kallikrein subfamily of serine proteases, which are involved in a variety of enzymatic processes. Its encoded protein is secreted and may play a role in suppression of tumorigenesis  in  breast  and  prostate cancers.KLK10  also contains 12 conserved cysteines in its protease domain that are expected to form 6 disulfide bonds, as in other serine proteases.  RT-PCR detected variable levels of  KLK10  expression in nearly all of the 35 adult and fetal tissues examined. The  KLK10  gene contains 6 exons, the first of which is noncoding. By fluorescence in situ hybridization,   Polikoff et al. (1997)   mapped the PRSSL1 gene to 19q13.3. By genomic sequence analysis,   Gan et al. (2000)   mapped the  KLK10  gene within the KLK gene cluster on chromosome 19q13. This cluster includes 13 KLK genes and 5 pseudogenes.

Synonyms

Kallikrein-10;3.4.21.-; Normal epithelial cell-specific 1; Protease serine-like 1; KLK10; NES1, PRSSL1

Gene Name

KLK10

Gene ID

5655

UniProt

O43240

Host

Rabbit

Reactivity

Human, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Kallikrein 10.

Clonality

Polyclonal

Tissue Specificity

Expressed in breast, ovary and prostate.

Applications

WB

Field of Research

Cancer, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Has a tumor-suppressor role for NES1 in breast and prostate cancer.

References & Citations

1. Elliott, M. B., Irwin, D. M., Diamandis, E. P. In silico identification and Bayesian phylogenetic analysis of multiple new mammalian kallikrein gene families. Genomics 88: 591-599, 2006. 2. Gan, L., Lee, I., Smith, R., Argonza-Barrett, R., Lei, H., McCuaig, J., Moss, P., Paeper, B., Wang, K. Sequencing and expression analysis of the serine protease gene cluster located in chromosome 19q13 region. Gene 257: 119-130, 2000. 3. Polikoff, D., Kuo, W.-L., Cochran, J. F., Wernick, M., Kowbel, D., Myambo, K., Collins, C. C. Assignment of protease, serine-like 1 (PRSSL1) to human chromosome 19q13 by in situ hybridization and radiation hybrid mapping. Cytogenet. Cell Genet. 79: 147-148, 1997.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

30170 MW

Observed Molecular Weight

37 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Kallikrein-10

Subcellular Location

Secreted .

Sequence Similarities

Belongs to the peptidase S1 family. Kallikrein subfamily.

Protein Name

Kallikrein-10

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.