Anti-Peroxiredoxin 2/PRDX2 Antibody Picoband®

About this product

Boster Bio Anti-Peroxiredoxin 2/PRDX2 Antibody catalog # PA1834. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1834

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

PRDX2 (peroxiredoxin 2) also known as NKEFB, PRP, PRX2, PRXII or TPX1, is a  protein  that in humans is encoded by the  PRDX2  gene. This gene encodes a member of the peroxiredoxin family of antioxidant  enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides.  The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8 (+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. The  Prdx2  -/- mice were healthy in appearance and fertile. Choi et al. (2005)   demonstrated that  PRDX2  is a negative regulator of PDGF signaling. Prx II deficiency results in increased production of peroxide, enhanced activation of PDGF receptor and phospholipase C-gamma-1, and subsequently increased cell proliferation and migration in response to PDGF. PRX2 accounted for about half of the thioredoxin activity in parasite extracts, and PRX2 expression was increased in the presence of chloroquine, regardless of P. falciparum strain susceptibility to the drug.&nbsp

Synonyms

Peroxiredoxin-2;1.11.1.15; Natural killer cell-enhancing factor B; NKEF-B; PRP; Thiol-specific antioxidant protein; TSA; Thioredoxin peroxidase 1; Thioredoxin-dependent peroxide reductase 1; PRDX2; NKEFB, TDPX1

Gene Name

PRDX2

Gene ID

7001

UniProt

P32119

Host

Rabbit

Reactivity

Rat, Mouse, Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Peroxiredoxin 2, identical to the related mouse and rat sequences.

Clonality

Polyclonal

Applications

WB

Field of Research

Alzheimer's Disease, Cancer, Cancer Metabolism, Cell Biology, Metabolism, Neurodegenerative Disease, Neurology Process, Neuroscience, Oxidative Stress, Pathways and Processes, Redox Metabolism

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system. It is not able to receive electrons from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H (2) O (2) .

References & Citations

1. Choi, M. H., Lee, I. K., Kim, G. W., Kim, B. U., Han, Y.-H., Yu, D.-Y., Park, H. S., Kim, K. Y., Lee, J. S., Choi, C., Bae, Y. S., Lee, B. I., Rhee, S. G., Kang, S. W. Regulation of PDGF signalling and vascular remodelling by peroxiredoxin II. Nature 435: 347-353, 2005. 2. Lee, T.-H., Kim, S.-U., Yu, S.-L., Kim, S. H., Park, D. S., Moon, H.-B., Dho, S. H., Kwon, K.-S., Kwon, H. J., Han, Y.-H., Jeong, S., Kang, S. W., Shin, H.-S., Lee, K.-K., Rhee, S. G., Yu, D.-Y. Peroxiredoxin II is essential for sustaining life span of erythrocytes in mice. Blood 101: 5033-5038, 2003. 3. Pahl, P., Berger, R., Hart, I., Chae, H. Z., Rhee, S. G., Patterson, D. Localization of TDPX1, a human homologue of the yeast thioredoxin-dependent peroxide reductase gene (TPX), to chromosome 13q12. Genomics 26: 602-606, 1995.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

21892 MW

Observed Molecular Weight

48-52 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Peroxiredoxin-2

Subcellular Location

Cytoplasm.

Sequence Similarities

Belongs to the AhpC/TSA family.

Protein Name

Peroxiredoxin-2

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.