Anti-Peroxiredoxin 3/PRDX3 Antibody Picoband®

About this product

Boster Bio Anti-Peroxiredoxin 3/PRDX3 Antibody catalog # PA1836. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1836

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

PRDX3 (peroxiredoxin 3) also known as AOP-1, MER5, SP-22 or PRX3, is localized exclusively in mitochondria. The deduced 256-amino acid human AOP1 protein shares 86% amino acid sequence similarity with mouse Aop1, and significant similarity with both the human proliferation-associated gene A product and the mouse stress-induced peritoneal macrophage protein Msp23.  The PRDX3 gene is mapped on 10q26.11. Expression of  PRDX3  is induced by MYC and is reduced in c-myc -/- cells. Chromatin immunoprecipitation analysis spanning the entire  PRDX3  genomic sequence revealed that MYC binds preferentially to a 930-bp region surrounding exon 1. Results using mitochondria-specific fluorescent probes demonstrated that  PRDX3  is essential for maintaining mitochondrial mass and membrane potential in transformed rat and human cells. These data provided evidence that  PRDX3  is a MYC target gene that is required to maintain normal mitochondrial function.

Synonyms

Thioredoxin-dependent peroxide reductase, mitochondrial;1.11.1.15; Antioxidant protein 1; AOP-1; PRX III; Perioredoxin-3; Protein MER5; Prdx3; Aop1, Mer5

Gene Name

PRDX3

Gene ID

11757

UniProt

P20108

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of mouse Peroxiredoxin 3, identical to the related rat sequence, and different from the related human sequence by three amino acids.

Clonality

Polyclonal

Tissue Specificity

Housekeeping-type gene preferentially expressed in murine erythroleukemia (MEL) cells.

Applications

IF, IHC, ICC, WB, Flow Cytometry

Field of Research

Apoptosis, Apoptotic Markers, Cancer, Cancer Metabolism, Cell Biology, Cell Death, Invasion/Microenvironment, Metabolism, Metabolism Processes, Mitochondrial, Mitochondrial Markers, Mitochondrial Metabolism, Oxidative Stress, Pathways and Processes, Redox Metabolism, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Involved in redox regulation of the cell. Protects radical-sensitive enzymes from oxidative damage by a radical- generating system.

References & Citations

1. Chiribau, C. B., Cheng, L., Cucoranu, I. C., Yu, Y.-S., Clempus, R. E., Sorescu, D. FOXO3A regulates peroxiredoxin III expression in human cardiac fibroblasts. J. Biol. Chem. 283: 8211-8217, 2008. 2. Nemoto, Y., Yamamoto, T., Takada, S., Matsui, Y., Obinata, M. Antisense RNA of the latent period gene (MER5) inhibits the differentiation of murine erythroleukemia cells. Gene 91: 261-265, 1990. 3. Wonsey, D. R., Zeller, K. I., Dang, C. V. The c-Myc target gene PRDX3 is required for mitochondrial homeostasis and neoplastic transformation. Proc. Nat. Acad. Sci. 99: 6649-6654, 2002.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

28127 MW

Observed Molecular Weight

26 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Thioredoxin-dependent peroxide reductase, mitochondrial

Subcellular Location

Mitochondrion .

Sequence Similarities

Belongs to the AhpC/TSA family.

Protein Name

Thioredoxin-dependent peroxide reductase, mitochondrial

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.