Anti-STAT5a Antibody Picoband®

About this product

Boster Bio Anti-STAT5a Antibody catalog # PA1840. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1840

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

STAT5 (Signal transducer and activator of transcription 5) also known as STAT5A, MGF, is a protein that serve the dual function of signal transducers and activators of transcription in cells exposed to signaling polypeptides. Hou et al. (1995)   cloned the human  STAT5  cDNA from an umbilical vein endothelial cell library and found that it encodes a 794-amino acid polypeptide with a predicted mass of approximately 90.5 kD. To analyze the possible role of  STAT5  in human GH-induced proliferation,   Friedrichsen et al. (2001)   expressed a dominant-negative  STAT5  mutant, STAT5A-delta-749, in INS-1 cells under the control of a doxycycline-inducible promoter by stable transfection.  Chromatin immunoprecipitation analysis confirmed binding of  STAT5  to the HIF2A promoter 344 bp upstream of the HIF2A start site.  STAT5  overexpression independently resulted in erythroid commitment in megakaryocytic-erythroid progenitors, which was abrogated by knockdown of GATA1.  STAT5  is responsible for the immediate-early induction of the long isoform of the BCL2-related protein in erythroid cells through binding to the promoter of the BCLX gene.&nbsp

Synonyms

Signal transducer and activator of transcription 5A; STAT5A; STAT5

Gene Name

STAT5A

Gene ID

6776

UniProt

P42229

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human STAT5a.

Clonality

Polyclonal

Tissue Specificity

Widely expressed. .

Applications

Flow Cytometry, WB

Field of Research

Epigenetics and Nuclear Signaling, Nuclear Signaling, Signal Transduction, Signaling Pathway, Transcription

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation. .

References & Citations

1. Friedrichsen, B. N., Galsgaard, E. D., Nielsen, J. H., Moldrup, A. Growth hormone- and prolactin-induced proliferation of insulinoma cells, INS-1, depends on activation of STAT5 (signal transducer and activator of transcription 5) . Molec. Endocr. 15: 136-148, 2001. 2. Hou, J., Schindler, U., Henzel, W. J., Wong, S. C., McKnight, S. L. Identification and purification of human Stat proteins activated in response to interleukin-2. Immunity 2: 321-329, 1995. 3. Schwaller, J., Parganas, E., Wang, D., Cain, D., Aster, J. C., Williams, I. R., Lee, C.-K., Gerthner, R., Kitamura, T., Frantsve, J., Anastasiadou, E., Loh, M. L., Levy, D. E., Ihle, J. N., Gilliland, D. G. Stat5 is essential for the myelo- and lymphoproliferative disease induced by TEL/JAK2.Molec. Cell 6: 693-704, 2000.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

90647 MW

Observed Molecular Weight

95 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Signal transducer and activator of transcription 5A

Subcellular Location

Cytoplasm . Nucleus . Translocated into the nucleus in response to phosphorylation.

Sequence Similarities

Belongs to the transcription factor STAT family.

Protein Name

Signal transducer and activator of transcription 5A

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.