- Catalog number
- PA1847
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Bellini-1 (beclin 1, autophagy related) is a protein that in humans is encoded by the BECN1 gene, also known as BECN1, ATG6, VPS30, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197, autophagy-related gene (Atg) 6. Beclin-1 and its binding partner class III phosphoinositide 3-kinase (PI3K), also named Vps34, are required for the initiation of the formation of the autophagosome in autophagy. Human beclin encodes a novel 450-amino acid protein containing a coiled-coil region, within which it has limited homology to myosin-like proteins. Northern blot analysis of mouse and adult human tissues revealed widespread beclin expression. A 2.2-kb transcript was present at highest levels in human skeletal muscle and at detectable levels in all tissues examined. In some tissues, additional 1.7- and 1.4-kb transcripts were observed, suggesting the presence of alternatively spliced transcripts. Immunoperoxidase staining of human hippocampus and frontal cortex sections revealed beclin immunoreactivity in many neurons throughout these regions, as well as in some glial cells. Beclin protects against infection by a neurovirulent strain of Sindbis virus that is known to overcome the protective effects of Bcl2. Liang et al. showed that beclin reduced Sindbis virus replication in mouse brain and reduced Sindbis virus-induced cell death in mouse brain.
- Synonyms
- Beclin-1; Coiled-coil myosin-like BCL2-interacting protein; Protein GT197; BECN1; GT197
- Gene Name
- BECN1
- Gene ID
- 8678
- UniProt
- Q14457
- Host
- Rabbit
- Reactivity
- Rat, Mouse, Human
- Cross Reactivity
- No cross-reactivity with other proteins
- Immunogen
- A synthetic peptide corresponding to a sequence in the middle region of human Beclin 1, identical to the related rat and mouse sequences.
- Clonality
- Polyclonal
- Tissue Specificity
- Ubiquitous.
- Applications
- WB
- Field of Research
- Cancer, Cancer Susceptibility, Cell Death, Epigenetics and Nuclear Signaling, Interspecies Interaction, Metabolism, Metabolism Processes, Microbiology, Oncoproteins/Suppressors, Pathways and Processes, Signal Transduction, Transcription, Tumor Suppressors
- Purification
- Immunogen affinity purified.
- Concentration
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Function
- Plays a central role in autophagy (PubMed:23184933) . Acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3- C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123, PubMed:20208530) . Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed:25275521) . Protects against infection by a neurovirulent strain of Sindbis virus (PubMed:9765397) . May play a role in antiviral host defense. .
- References & Citations
- 1. Liang, X. H., Kleeman, L. K., Jiang, H. H., Gordon, G., Goldman, J. E., Berry, G., Herman, B., Levine, B.Protection against fatal Sindbis virus encephalitis by beclin, a novel Bcl-2-interacting protein. J. Virol. 72: 8586-8596, 1998.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 51896 MW
- Observed Molecular Weight
- 46 kDa
- Specificity
- No cross reactivity with other proteins.
- Gene Name Synonym
- Beclin-1
- Subcellular Location
- Cytoplasm . Golgi apparatus, trans-Golgi network membrane ; Peripheral membrane protein . Endosome membrane ; Peripheral membrane protein . Endoplasmic reticulum membrane ; Peripheral membrane protein . Mitochondrion membrane ; Peripheral membrane protein . Endosome . Cytoplasmic vesicle, autophagosome . Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells (By similarity) . .
- Protein Name
- Beclin-1
- Isotype
- Rabbit IgG
- Contents
- Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
