Anti-Hyaluronidase-3 HYAL3 Antibody Picoband®

About this product

Boster Bio Anti-Hyaluronidase-3 HYAL3 Antibody catalog # PA1856. Tested in IHC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1856

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

HYAL3 (hyaluronoglucosaminidase 3) is an  enzyme  that in humans is encoded by the  HYAL3 gene, also known as LUCA3, Hyaluronidase-3, Lung carcinoma protein 3, LUCA-3, LUCA14, Minna14. By Northern blot analysis,   expression of a 2.1-kb HYAL3 transcript that was strongest in testis and bone marrow, but relatively weak in other organs. HYAL3 encodes a predicted 417-amino acid protein. This gene encodes a protein which is similar in structure to hyaluronidases. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is though to be involved in cell proliferation, migration and differentiation. However, this protein has not yet been shown to have hyaluronidase activity. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression.

Synonyms

Hyaluronidase-3; Hyal-3;3.2.1.35; Hyaluronoglucosaminidase-3; Lung carcinoma protein 3; LuCa-3; HYAL3; LUCA3

Gene Name

HYAL3

Gene ID

8372

UniProt

O43820

Host

Rabbit

Reactivity

Mouse, Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human HYAL3, different from the related rat sequence by three amino acids, and different from the related mouse sequence by one amino acid.

Clonality

Polyclonal

Tissue Specificity

Bone marrow, testis and kidney. Isoform 4 is detected in all bladder tumor and prostate tumor cells. .

Applications

IHC, WB

Field of Research

Cancer, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Tyrosine-protein kinase that acts downstream of cell surface receptors and plays a role in the regulation of the actin cytoskeleton, microtubule assembly, cell attachment and cell spreading. Plays a role in FCER1 (high affinity immunoglobulin epsilon receptor) -mediated signaling in mast cells. Acts down- stream of the activated FCER1 receptor and the mast/stem cell growth factor receptor KIT. Plays a role in the regulation of mast cell degranulation. Plays a role in the regulation of cell differentiation and promotes neurite outgrowth in response to NGF signaling. Plays a role in cell scattering and cell migration in response to HGF-induced activation of EZR. Phosphorylates BCR and down-regulates BCR kinase activity. Phosphorylates HCLS1/HS1, PECAM1, STAT3 and TRIM28. .

References & Citations

1. Csoka, A. B., Scherer, S. W., Stern, R. Expression analysis of six paralogous human hyaluronidase genes clustered on chromosomes 3p21 and 7q31.Genomics 60: 356-361, 1999.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

46501 MW

Observed Molecular Weight

93 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Hyaluronidase-3

Subcellular Location

Secreted . Lysosome .

Sequence Similarities

Belongs to the glycosyl hydrolase 56 family.

Protein Name

Hyaluronidase-3 (Hyal-3)

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.