Anti-SHP2/PTPN11 Antibody Picoband®

About this product

Boster Bio Anti-SHP2/PTPN11 Antibody catalog # PA1860. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1860

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

PTPN11 (Tyrosine-protein phosphatase non-receptor type 11), also known as protein-tyrosine phosphatase 1D (PTP-1D),   protein-tyrosine phosphatase 2C (PTP-2C), TYROSINE PHOSPHATASE SHP2 (SHP2), BPTP3, SH-PTP2, SHP-2, SH-PTP3, is an  enzyme  that in humans is encoded by the  PTPN11  gene. PTPN11 is a member of the protein tyrosine phosphatase (PTP) family. The open reading frame consists of 1,779 nucleotides potentially encoding a protein of 593 amino acids with a predicted molecular mass of 68 kD. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of  Noonan syndrome  as well as acute myeloid leukemia.

Synonyms

Tyrosine-protein phosphatase non-receptor type 11;3.1.3.48; Protein-tyrosine phosphatase 1D; PTP-1D; Protein-tyrosine phosphatase 2C; PTP-2C; SH-PTP2; SHP-2; Shp2; SH-PTP3; PTPN11; PTP2C, SHPTP2

Gene Name

PTPN11

Gene ID

5781

UniProt

Q06124

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human SHP2, different from the related rat and mouse sequences by one amino acid.

Clonality

Polyclonal

Tissue Specificity

Widely expressed, with highest levels in heart, brain, and skeletal muscle. .

Applications

IF, IHC, ICC, WB, Flow Cytometry

Field of Research

Auditory System, Neuroscience, Protein Phosphorylation, Sensory System, Signal Transduction, Tyrosine Phosphatases

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Dephosphorylates ROCK2 at Tyr-722 resulting in stimulatation of its RhoA binding activity. .

References & Citations

1. Higashi, H., Tsutsumi, R., Muto, S., Sugiyama, T., Azuma, T, Asaka, M., Hatakeyama, M. SHP-2 tyrosine phosphatase as an Fixed target of Helicobacter pylori CagA protein. Science 295: 683-686, 2002.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

68436 MW

Observed Molecular Weight

68 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Tyrosine-protein phosphatase non-receptor type 11

Subcellular Location

Cytoplasm.

Sequence Similarities

Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.

Protein Name

Tyrosine-protein phosphatase non-receptor type 11

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.