Anti-CD1a Antibody Picoband®

About this product

Boster Bio Anti-CD1a Antibody catalog # PA1875. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1875

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD1A (Thymocyte antigen CD1A) also known as CD1, is a human  protein  encoded by the  CD1A gene. CD1A  is 1 of 5 distinct CD1 genes that are variably conserved in different mammalian species.  This gene encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin.The CD1A gene is mapped on 1q23.1. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells.  CD1A  is concentrated in the early or recycling endosome. Moody et al. (2004)   reported that  CD1A  presents to T cells a family of previously unknown lipopeptides from Mycobacterium tuberculosis, named didehydroxymycobactins because of their structural relation to mycobactin siderophores.  The protein encoded by this gene localizes to the plasma membrane and to recycling vesicles of the early endocytic system. Apolipoprotein E (107741) binds lipid antigens and delivers them by receptor-mediated uptake into endosomal compartments containing CD1 in antigen-presenting cells.

Synonyms

T-cell surface glycoprotein CD1a; T-cell surface antigen T6/Leu-6; hTa1 thymocyte antigen; CD1a; CD1A

Gene Name

CD1A

Gene ID

909

UniProt

P06126

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human CD1a.

Clonality

Polyclonal

Tissue Specificity

Expressed on cortical thymocytes, epidermal Langerhans cells, dendritic cells, on certain T-cell leukemias, and in various other tissues. .

Applications

WB

Field of Research

Adaptive Immunity, Immunology, T Cells

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells. .

References & Citations

1. Martin, L. H., Calabi, F., Lefebvre, F.-A., Bilsland, C. A. G., Milstein, C. Structure and expression of the human thymocyte antigens CD1a, CD1b, and CD1c.Proc. Nat. Acad. Sci. 84: 9189-9193, 1987. 2. Moody, D. B., Young, D. C., Cheng, T.-Y., Rosat, J.-P., Roura-mir, C., O'Connor, P. B., Zajonc, D. M., Walz, A., Miller, M. J., Levery, S. B., Wilson, I. A., Costello, C. E., Brenner, M. B. T cell activation by lipopeptide antigens. Science 303: 527-531, 2004. Note: Erratum: Science 304: 211 only, 2004. 3. Park, S.-H., Bendelac, A. CD1-restricted T-cell responses and microbial infection. Nature 406: 788-792, 2000.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

37077 MW

Observed Molecular Weight

37 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

T-cell surface glycoprotein CD1a

Subcellular Location

Cell membrane; Single-pass type I membrane protein. Endosome membrane. Subject to intracellular trafficking between the cell membrane and endosomes. Localizes to cell surface lipid rafts.

Sequence Similarities

Contains 9 SRCR domains.

Protein Name

T-cell surface glycoprotein CD1a

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.