Anti-Krueppel-like factor 3 KLF3 Antibody Picoband®

About this product

Boster Bio Anti-Krueppel-like factor 3 KLF3 Antibody catalog # PA1888. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1888

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

KLF3 (Krueppel-like factor 3) is a protein that in humans is encoded by the KLF3 gene. It is also known as BKLF (Basic kruppel-like factor) . KLF3 is a member of the Krü ppel-like factors family of transcription factors. KLF3 primarily represses transcription through the transcriptional corepressor C-terminal binding protein (CtBP) . Suske et al. (2005) stated that the human KLF3 gene maps to chromosome 4p14, and the mouse Klf3 gene maps to chromosome 5C3.3. Suske et al. (2005) stated that the human and mouse KLF3 proteins contain 345 and 344 amino acids, respectively. Both have a characteristic zinc finger domain, but lack the N-terminal buttonhead box found in related SP transcription factors. The repression mediated through KLF3 is also partially dependent on the sumoylation of its K10 and K197. Within KLF3's C-terminal, which is highly conserved throughout the KLF family, resides three C2H2 zinc fingers that preferentially bind the site NRG GNG NGR.

Synonyms

Krueppel-like factor 3; Basic krueppel-like factor; CACCC-box-binding protein BKLF; TEF-2; KLF3; BKLF

Gene Name

KLF3

Gene ID

51274

UniProt

P57682

Host

Rabbit

Reactivity

Rat, Mouse, Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human KLF3, different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino acids.

Clonality

Polyclonal

Applications

WB

Field of Research

Cardiovascular, Cell Biology, Cell Cycle, Cell Differentiation, Domain Families, Epigenetics and Nuclear Signaling, Krueppel Like Factor, Transcription, Transcription Factors

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Binds to the CACCC box of erythroid cell-expressed genes. May play a role in hematopoiesis (By similarity) . .

References & Citations

1. Suske, G., Bruford, E., Philipsen, S.Mammalian SP/KLF transcription factors: bring in the family.Genomics 85: 551-556, 2005. 2. Turner, Jeremy; Nicholas Hannah, Bishop David, Matthews Jacqueline M, Crossley Merlin The LIM protein FHL3 binds basic Krüppel-like factor/Krüppel-like factor 3 and its co-repressor C-terminal-binding protein 2. J. Biol. Chem. (United States) 278 (15) : 12786–95. 2003 3. Wang MJ, Qu XH, Wang LS, et al.. [cDNA cloning, subcellular localization and tissue expression of a new human Krüppel-like transcription factor: human basic Krüppel-like factor (hBKLF) ]. Yi Chuan Xue Bao 30 (1) : 1–9. 2003.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

38829 MW

Observed Molecular Weight

18 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Krueppel-like factor 3

Subcellular Location

Nucleus .

Sequence Similarities

Belongs to the krueppel C2H2-type zinc-finger protein family.

Protein Name

Krueppel-like factor 3

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.