- Catalog number
- PA1897
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- SHC1 (SHC-transforming protein 1), also known as SHCA, is a protein that in humans is encoded by the SHC1 gene. SCOP classifies the 3D structure as belonging to the SH2 domain family. By Southern analysis of somatic cell hybrids followed by both isotopic and fluorescence in situ hybridization, Huebner et al. (1994) assigned the SHC1 gene to 1q21. Yulug et al. (1995) used fluorescence in situ hybridization to map the SHC1 gene to 1q21. By the same method, an SHC-related sequence (SHCL1) was mapped to 17q21-q22. By FISH analysis and sequencing of vectorette library PCR products, Harun et al. (1997) identified SHC1P1, a 3.2-kb processed pseudogene, in Xq12-q13.1. SHC1P1 is 85% identical to mouse Shc p66. Reporter assays showed FKHRL1 transactivates CAT, suggesting a capacity to augment antioxidant scavenging. Nemoto and Finkel (2002) concluded that there is an important functional relationship between forkhead proteins (e.g., FKHRL1), SHC1, and intracellular oxidants, all of which are though to be involved in the aging process in worms and mammals.
- Synonyms
- SHC-transforming protein 1; SHC-transforming protein 3; SHC-transforming protein A; Src homology 2 domain-containing-transforming protein C1; SH2 domain protein C1; SHC1; SHC, SHCA
- Gene Name
- SHC1
- Gene ID
- 6464
- UniProt
- P29353
- Host
- Rabbit
- Reactivity
- Human, Mouse, Rat
- Cross Reactivity
- No cross-reactivity with other proteins
- Immunogen
- A synthetic peptide corresponding to a sequence at the C-terminus of human SHC, different from the related rat and mouse sequences by two amino acids.
- Clonality
- Polyclonal
- Tissue Specificity
- Widely expressed. Expressed in neural stem cells but absent in mature neurons.
- Applications
- IHC, IHC-F, ICC, WB
- Field of Research
- Adapters, Alzheimer's Disease, Cytoplasmic, Neurodegenerative Disease, Neurology Process, Neuroscience, Signal Transduction
- Purification
- Immunogen affinity purified.
- Concentration
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Function
- Signaling adapter that couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span (By similarity) . Participates in signaling downstream of the angiopoietin receptor TEK/TIE2, and plays a role in the regulation of endothelial cell migration and sprouting angiogenesis. .
- References & Citations
- 1. Harun, R. B., Smith, K. K., Leek, J. P., Markham, A. F., Norris, A., Morrison, J. F. J.Characterization of human SHC p66 cDNA and its processed pseudogene mapping to Xq12-q13.1.Genomics 42: 349-352, 1997. 2. Huebner, K., Kastury, K., Druck, T., Salcini, A. E., Lanfrancone, L., Pelicci, G., Lowenstein, E., Li, W., Park, S.-H., Cannizzaro, L., Pelicci, P. G., Schlessinger, J.Chromosome locations of genes encoding human signal transduction adapter proteins, Nck (NCK), Shc (SHC1), and Grb2 (GRB2) .Genomics 22: 281-287, 1994. 3. Pelicci, G., Lanfrancone, L., Grignani, F., McGlade, J., Cavallo, F., Forni, G., Nicoletti, I., Grignani, F., Pawson, T., Pelicci, P. G.A novel transforming protein (SHC) with an SH2 domain is implicated in mitogenic signal transduction.Cell 70: 93-104, 1992.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 62822 MW
- Observed Molecular Weight
- 46 kDa, 52 kDa, 66 kDa
- Specificity
- No cross reactivity with other proteins.
- Gene Name Synonym
- SHC-transforming protein 1
- Subcellular Location
- Cytoplasm.
- Sequence Similarities
- Contains 1 PID domain.
- Protein Name
- SHC-transforming protein 1
- Isotype
- Rabbit IgG
- Contents
- Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
