Anti-NOXA1 Antibody Picoband®

About this product

Boster Bio Anti-NOXA1 Antibody catalog # PA1930. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1930

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

NOXA1 (NADPH oxidase activator 1), also called NOX ACTIVATOR 1 or p51-NOX, is an enzyme that in humans is encoded by the NOXA1 gene. Hartz (2007) mapped the NOXA1 gene to chromosome 9q34.3 based on an alignment of the NOXA1 sequence with the genomic sequence (build 36.1) . Banfi et al. (2003) mapped the mouse Noxa1 gene to chromosome 2. Using yeast 2-hybrid assays, Takeya et al. (2003) showed that human p51-NOX interacted with constitutively active forms of RAC1 and RAC2. In vitro binding assays revealed that p51-NOX bound GTP-bound RAC1, but not GDP-bound RAC1. p51-NOX also bound p47-PHOX (NCF1) and p41-NOX (NOXO1), and trp436 within the SH3 domain of p51-NOX was required for these interactions. Human cell lines or COS-7 cells cotransfected with p51-NOX and p41-NOX and either gp91-PHOX (CYBB) or NOX1 produced superoxide. Cells individually transfected with NOX1, p41-NOX, or p51-NOX and cells transfected with only p41-NOX and p51-NOX showed no superoxide production.

Synonyms

NADPH oxidase activator 1; NOX activator 1; Antigen NY-CO-31; NCF2-like protein; P67phox-like factor; p51-nox; NOXA1; P51NOX

Gene Name

NOXA1

Gene ID

10811

UniProt

Q86UR1

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human NOXA1.

Clonality

Polyclonal

Tissue Specificity

Widely expressed. Detected in pancreas, liver, kidney, spleen, prostate, small intestine and colon. .

Applications

WB

Field of Research

Associated Proteins, Cardiovascular, Cell Biology, Hypoxia, Metabolism, Organelle Proteins, Oxidative Stress, Pathways and Processes, Protein Trafficking, Redox Metabolism, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Functions as an activator of NOX1, a superoxide- producing NADPH oxidase. Functions in the production of reactive oxygen species (ROS) which participate in a variety of biological processes including host defense, hormone biosynthesis, oxygen sensing and signal transduction. May also activate CYBB/gp91phox and NOX3. .

References & Citations

1. Banfi, B., Clark, R. A., Steger, K., Krause, K.-H. Two novel proteins activate superoxide generation by the NADPH oxidase NOX1. J. Biol. Chem. 278: 3510-3513, 2003. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 7/25/2007. 3. Takeya, R., Ueno, N., Kami, K., Taura, M., Kohjima, M., Izaki, T., Nunoi, H., Sumimoto, H. Novel human homologues of p47 (phox) and p67 (phox) participate in activation of superoxide-producing NADPH oxidases. J. Biol. Chem. 278: 25234-25246, 2003.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

51 kDa

Observed Molecular Weight

60 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

NADPH oxidase activator 1

Subcellular Location

Cytoplasm. Cell membrane. Translocation to membranes depends on NOXO1 or NCF1 and maybe RAC1.

Sequence Similarities

Belongs to the NCF2/NOXA1 family.

Protein Name

NADPH oxidase activator 1

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.