Anti-Nuclear respiratory factor 1 NRF1 Antibody Picoband®

About this product

Boster Bio Anti-Nuclear respiratory factor 1 NRF1 Antibody catalog # PA1948. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1948

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Nuclear respiratory factor 1, is also known as NRF1. This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternative splicing results in multiple transcript variants. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for nuclear factor (erythroid-derived 2) -like 1 which has an official symbol of NFE2L1.

Synonyms

Nuclear respiratory factor 1; NRF-1; Alpha palindromic-binding protein; Alpha-pal; NRF1

Gene Name

NRF1

Gene ID

4899

UniProt

Q16656

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human NRF1, identical to the related rat and mouse sequences.

Clonality

Polyclonal

Tissue Specificity

Ubiquitously expressed with strongest expression in skeletal muscle.

Applications

IHC, ICC, WB

Field of Research

Epigenetics and Nuclear Signaling, Metabolic Signaling Pathways, Metabolism, Mitochondrial Metabolism, Nucleotide Metabolism, Pathways and Processes, Pol II Transcription, Polymerase Associated Factors, Transcription, Transcription Factors

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication.

References & Citations

1. Efiok, B. J. S., Chiorini, J. A., Safer, B. A key transcription factor for eukaryotic initiation factor-2-alpha is strongly homologous to developmental transcription factors and may link metabolic genes to cellular growth and development. J. Biol. Chem. 269: 18921-18930, 1994. 2. Gopalakrishnan, L., Scarpulla, R. C. Structure, expression, and chromosomal assignment of the human gene encoding nuclear respiratory factor 1. J. Biol. Chem. 270: 18019-18025, 1995. 3. Virbasius, J. V., Scarpulla, R. C. Activation of the human mitochondrial transcription factor A gene by nuclear respiratory factors: a potential regulatory link between nuclear and mitochondrial gene expression in organelle biogenesis. Proc. Nat. Acad. Sci. 91: 1309-1313, 1994.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

53541 MW

Observed Molecular Weight

75 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Nuclear respiratory factor 1

Subcellular Location

Nucleus.

Sequence Similarities

Belongs to the NRF1/Ewg family.

Protein Name

Nuclear respiratory factor 1

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.