Anti-REA/PHB2 Antibody Picoband®

About this product

Boster Bio Anti-REA/PHB2 Antibody catalog # PA1951. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1951

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Reporter gene

Synonyms

Prohibitin-2; B-cell receptor-associated protein BAP37; D-prohibitin; Repressor of estrogen receptor activity; PHB2 ; BAP , REA

Gene Name

PHB2

Gene ID

11331

UniProt

Q99623

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human REA, identical to the related rat and mouse sequences.

Clonality

Polyclonal

Tissue Specificity

Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U-937 histiocytic lymphoma cell lines (at protein level) . .

Applications

IHC, ICC, WB

Field of Research

Chaperones, Co-Activators/Co-Repressors, Epigenetics and Nuclear Signaling, Growth Factors/Hormones, Nuclear Hormone Receptors, Nuclear Receptors, Nuclear Signaling, Nuclear Signaling Pathways, Protein Trafficking, Signal Transduction, Signaling Pathway

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases (By similarity) . Functions as an estrogen receptor (ER) -selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. Competes with NCOA1 for modulation of ER transcriptional activity. Probably involved in regulating mitochondrial respiration activity and in aging. .

References & Citations

1. Merkwirth, C., Dargazanli, S., Tatsuta, T., Geimer, S., Lower, B., Wunderlich, F. T., von Kleist-Retzow, J.-C., Waisman, A., Westermann, B., Langer, T. Prohibitins control cell proliferation and apoptosis by regulating OPA1-dependent cristae morphogenesis in mitochondria. Genes Dev. 22: 476-488, 2008. 2. Montano, M. M., Ekena, K., Delage-Mourroux, R., Chang, W., Martini, P., Katzenellenbogen, B. S. An estrogen receptor-selective coregulator that potentiates the effectiveness of antiestrogens and represses the activity of estrogens. Proc. Nat. Acad. Sci. 96: 6947-6952, 1999. 3. Mussi, P., Liao, L., Park, S.-E., Ciana, P., Maggi, A., Katzenellenbogen, B. S., Xu, J., O'Malley, B. W. Haploinsufficiency of the corepressor of estrogen receptor activity (REA) enhances estrogen receptor function in the mammary gland. Proc. Nat. Acad. Sci. 103: 16716-16721, 2006.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

33296 MW

Observed Molecular Weight

120 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Prohibitin-2

Subcellular Location

Mitochondrion inner membrane . Cytoplasm . Nucleus . Also cytoplasmic and nuclear. .

Sequence Similarities

Contains 6 Ig-like C2-type (immunoglobulin-like) domains.

Protein Name

Prohibitin-2

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.